GABBR1 Antikörper (C-Term)

Produktnummer ABIN1098201

Produktdetails anti-GABBR1 Antikörper

Target: GABBR1 (gamma-aminobutyric Acid (GABA) B Receptor, 1 (GABBR1))

Bindungsspezifität: AA 873-977, C-Term

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GABBR1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (fixed cells) (IF/ICC)

Verwendungszweck: Anti-GABA (B)R1 Mouse Monoclonal Antibody

Spezifität: This antibody recognizes human, mouse, and rat GABA(B)R1. It does not cross- react with GABA(B)R2.

Kreuzreaktivität: Human, Maus, Ratte

Kreuzreaktivität (Details): No cross Reactivity with GABA(B)R2

Aufreinigung: Purified by Protein G affinity chromatography.

Immunogen: Fusion protein corresponding to aa 873-977 (cytoplasmic C-terminus) of rat GABA(B)R1 (accession no. NP_112290.2).

Klon: S93A-49

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunoblotting: use at 1-2 μg/mL. A band of ~115 kDa is detected.
Immunocytochemistry: use at 1-5 μg/mL.
These are recommended concentrations.
Enduser should determine optimal concentrations for their applications.
Positive control: Rat brain membranes.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Dilute in PBS or medium that is identical to that used in the assay system.

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 50 % glycerol, 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: This product is stable for at least 1 year at -20°C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles.

Details zu GABBR1

Target: GABBR1 (gamma-aminobutyric Acid (GABA) B Receptor, 1 (GABBR1))

Andere Bezeichnung: GABA(B)R1 (GABBR1 Produkte)

Synonyme: GABABR1 antikoerper, GABBR1-3 antikoerper, GB1 antikoerper, GPRC3A antikoerper, dJ271M21.1.1 antikoerper, dJ271M21.1.2 antikoerper, gabbr1 antikoerper, GABBR1 antikoerper, GABA(B)R1 antikoerper, GABAB1 antikoerper, GABAbR1 antikoerper, bM573K1.1 antikoerper, gamma-aminobutyric acid type B receptor subunit 1 antikoerper, gamma-aminobutyric acid (GABA) B receptor, 1a antikoerper, gamma-aminobutyric acid (GABA) B receptor, 1 antikoerper, GABBR1 antikoerper, gabbr1a antikoerper, gabbr1 antikoerper, Gabbr1 antikoerper

Hintergrund: γ-aminobutyric acid type B receptor subunit 1,Component of a heterodimeric G-protein coupled receptor for GABA, formed by GABBR1 and GABBR2 (PubMed:9872315, PubMed:9872317, PubMed:9872744). Within the heterodimeric GABA receptor, only GABBR1 seems to bind agonists, while GABBR2 mediates coupling to G proteins (PubMed:9872317, PubMed:10658574). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase (PubMed:10075644, PubMed:9872315, PubMed:9872744, PubMed:10924501). Signaling inhibits adenylate cyclase, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipid hydrolysis (PubMed:9069281, PubMed:10457184, PubMed:9872315, PubMed:9872744, PubMed:10924501, PubMed:10692480). Calcium is required for high affinity binding to GABA (PubMed:10692480). Plays a critical role in the fine-tuning of inhibitory synaptic transmission (PubMed:9872744). Pre-synaptic GABA receptor inhibits neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA receptor decreases neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials (PubMed:9872744, PubMed:10924501, PubMed:10692480). Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception (By similarity). {UniProtKB:Q9UBS5, PubMed:10075644, PubMed:10457184, PubMed:10658574, PubMed:10692480, PubMed:10924501, PubMed:9069281, PubMed:9872315, PubMed:9872744}.,GABA (g-aminobutyric acid) is the primary inhibitory neurotransmitter in the central nervous system and interacts with three different receptors: GABA(A), GABA(B), and GABA(C). GABA(B) receptor is coupled to G proteins that modulate slow inhibitory synaptic transmission. Functional GABA(B) receptors form heterodimers of GABA(B)R1 and GABA(B)R2 in which GABA(B)R1 binds a ligand and GABA(B)R2 is the primary G protein contact site.,Cell membrane, Cell junction, synapse, postsynaptic cell membrane, Cell projection, dendrite, Perikaryon,GABA-B receptor 1, GABA-B-R1, GABA-BR1, GABABR1, Gb1

UniProt: Q9Z0U4

Pathways: Positive Regulation of Peptide Hormone Secretion, cAMP Metabolic Process