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Calmodulin,CAM Antikörper (AA 1-149)

Reaktivität: Ratte WB, ICC, ELISA, IHC (fro), IHC (p) Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN1077890
  • Target Alle Calmodulin,CAM Antikörper anzeigen
    Calmodulin,CAM
    Bindungsspezifität
    AA 1-149
    Reaktivität
    Ratte
    Wirt
    • 3
    Kaninchen
    Klonalität
    • 3
    Polyklonal
    Konjugat
    • 1
    • 1
    • 1
    Dieser Calmodulin,CAM Antikörper ist unkonjugiert
    Applikation
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunocytochemistry (ICC), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Sequenz
    MGHHHHHHSG S- MADQLTEEQI AEFKEAFSLF DKDGDGTITT KELGTVMRSL GQNPTEAELQ DMINEVDADG NGTIDFPEFL TMMARKMKDT DSEEEIREAF RVFDKDGNGY ISAAELRHVM TNLGEKLTDE EVDEMIREAD IDGDGQVNYE EFVQMMTAK
    Spezifität
    It has been selected for its ability to recognize CAM in immunohistochemical staining and Western blotting.
    Aufreinigung
    Affinity Chromatography
    Immunogen
    Recombinant CAM expressed in E.coli.
    The antibody is a rabbit polyclonal antibody raised against CAM.
    Isotyp
    IgG
  • Applikationshinweise
    Western blotting: 1:100-400
    Immunocytochemistry in formalin fixed cells: 1:100-500
    Immunohistochemistry in formalin fixed frozen section: 1:100-500
    Immunohistochemistry in paraffin section: 1:50-200
    Enzyme-linked Immunosorbent Assay: 1:100-200
    Optimal working dilutions must be determined by end user.
    Kommentare

    Content: The quality control contains recombinant CAM (Met1~Lys149) disposed in loading buffer.
    Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
    Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
    Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    Lot specific
    Buffer
    Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Handhabung
    Avoid repeated freeze/thaw cycles
    Lagerung
    4 °C
    Informationen zur Lagerung
    Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
    Haltbarkeit
    12 months
  • Target
    Calmodulin,CAM
    Andere Bezeichnung
    Calmodulin (CAM) (Calmodulin,CAM Produkte)
    Synonyme
    CALM2 antikoerper, CaMA antikoerper, R75142 antikoerper, PHKD antikoerper, PHKD3 antikoerper, calmodulin 1 antikoerper, calmodulin 2 antikoerper, calmodulin 3 antikoerper, CALM1 antikoerper, Calm2 antikoerper, CALM2 antikoerper, Calm3 antikoerper, CALM3 antikoerper
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