SUMO Antikörper

Produktnummer ABIN107152

Produktdetails anti-SUMO Antikörper

Target: SUMO

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SUMO Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Produktmerkmale: Concentration Definition: by UV absorbance at 280 nm

Immunogen: This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant human SUMO protein.
Immunogentype:Recombinant

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: This purified polyclonal antibody reacts with human SUMO by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.   This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or conjugates).  Other intrinsic bands are readily detectable at lower dilutions. For immunoblotting a 1:2,000 dilution is recommended.  An 11.6 kDa band corresponding to human SUMO is detected.  Most human cell lysates can be used as a positive control without induction or stimulation.  For ELISA a 1:4,000 to 1:20,000 dilution is recommended.   Researchers should determine optimal titers for other applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Restore with deionized water (or equivalent)

Konzentration: 5.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Referenzen für anti-SUMO Antikörper (ABIN107152)

Meyer, Shah, Zhang, Rohrs, Rao: "Evidence for intermolecular interactions between the intracellular domains of the arabidopsis receptor-like kinase ACR4, its homologs and the Wox5 transcription factor." in: PLoS ONE, Vol. 10, Issue 3, pp. e0118861, (2016) (PubMed).

Details zu SUMO

Target: SUMO

Abstract: SUMO Produkte

Synonyme: CG4494 antikoerper, Dm0342 antikoerper, DmSUMO-1 antikoerper, DmSmt3 antikoerper, Dmel\\CG4494 antikoerper, Dmsmt3 antikoerper, SMT3 antikoerper, SUMO antikoerper, Smt3 antikoerper, Sumo antikoerper, anon-EST:Posey240 antikoerper, dSmt3 antikoerper, dsmt3 antikoerper, l(2)04493 antikoerper, l(2)SH0182 antikoerper, l(2)SH2 0182 antikoerper, sumo antikoerper, CG4494 gene product from transcript CG4494-RA antikoerper, smt3 antikoerper

Hintergrund: Covalent modification of cellular proteins by the ubiquitin-like modifier SUMO (small ubiquitin-like modifier) regulates various cellular processes, such as nuclear transport, signal transduction, stress responses and cell cycle progression.  But, in contrast to ubiquination, sumoylation does not tag proteins for degradation by the 26S proteasome, but rather seems to enhance stability or modulate their subcellular compartmentalization.  Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin.  Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12.  Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs).  These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other.  In contrast to UBLs, UDPs are not conjugated to other proteins.  Once covalently attached to cellular targets, SUMO regulates protein:protein and protein:DNA interactions, as well as localization and stability of the target protein.  Sumoylation occurs in most eukaryotic systems, and SUMO is highly conserved from yeast to humans.   Where invertebrates have only a single SUMO gene termed SMT3, three members of the SUMO family have been identified in
Synonyms: GAP modifying protein 1 antibody, GMP 1 antibody, GMP1 antibody, PIC 1 antibody, PIC1 antibody, SENP2 antibody, Sentrin 1 antibody, Sentrin antibody, Small ubiquitin related modifier 1 antibody

Gen-ID: 7341

UniProt: P63165