MMP13 Antikörper (Internal Region)

Produktnummer ABIN1049066

Produktdetails anti-MMP13 Antikörper

Target: MMP13 (Matrix Metallopeptidase 13 (Collagenase 3) (MMP13))

Bindungsspezifität: Internal Region

Reaktivität: Human, Kaninchen, Schwein, Affe, Pferd

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MMP13 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human MMP13. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Elephant, Panda, Horse, Rabbit, Pig (100%) Mouse, Rat, Sheep, Dog, Hamster (94%) Bovine, Opossum, Turkey (89%) Chicken (83%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 18 amino acid peptide from internal region of human MMP13. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Elephant, Panda, Horse, Rabbit, Pig (100%), Mouse, Rat, Sheep, Dog, Hamster (94%), Bovine, Opossum, Turkey (89%), Chicken (83%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (15 - 25 μg/mL)

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu MMP13

Target: MMP13 (Matrix Metallopeptidase 13 (Collagenase 3) (MMP13))

Andere Bezeichnung: MMP13 (MMP13 Produkte)

Synonyme: CLG3 antikoerper, MANDP1 antikoerper, Clg antikoerper, MMP-13 antikoerper, Mmp1 antikoerper, cb1034 antikoerper, mmp13 antikoerper, gene A antikoerper, xCol antikoerper, xcl3 antikoerper, MMP13 antikoerper, MGC108008 antikoerper, matrix metallopeptidase 13 antikoerper, matrix metallopeptidase 13a antikoerper, matrix metallopeptidase 13 (collagenase 3) like S homeolog antikoerper, matrix metallopeptidase 13 (collagenase 3) antikoerper, matrix metalloproteinase 13 antikoerper, matrix metallopeptidase 13 (collagenase 3) like L homeolog antikoerper, MMP13 antikoerper, Mmp13 antikoerper, mmp13a antikoerper, mmp13l.S antikoerper, mmp13 antikoerper, LOC100136348 antikoerper, mmp13l.L antikoerper

Hintergrund: Name/Gene ID: MMP13
Subfamily: Metallopeptidase M10A
Family: Protease

Synonyms: MMP13, Collagenase 3, Matrix metalloproteinase-13, Interstitial collagenase, MANDP1, MMP-13, CLG3

Gen-ID: 4322