Dopamine d2 Receptor Antikörper (Cytoplasmic Domain)

Produktnummer ABIN1048503

Produktdetails anti-Dopamine d2 Receptor Antikörper

Target: Dopamine d2 Receptor (DRD2) (Dopamine Receptor D2 (DRD2))

Bindungsspezifität: Cytoplasmic Domain

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Dopamine d2 Receptor Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human DRD2. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except ZNF57 (42 % ).

Homologie: Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Marmoset (100%) Gibbon, Monkey, Rabbit (95%) Dog, Bovine, Panda, Horse, Pig (89%) Mouse, Rat, Ferret, Bat, Hamster, Elephant (84%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 19 amino acid peptide from 3rd cytoplasmic domain of human DRD2. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Marmoset (100%), Gibbon, Monkey, Rabbit (95%), Dog, Bovine, Panda, Horse, Pig (89%), Mouse, Rat, Ferret, Bat, Hamster, Elephant (84%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 - 10 μg/mL)

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu Dopamine d2 Receptor

Target: Dopamine d2 Receptor (DRD2) (Dopamine Receptor D2 (DRD2))

Andere Bezeichnung: DRD2 / Dopamine Receptor D2 (DRD2 Produkte)

Synonyme: D2DR antikoerper, D2R antikoerper, drd2b antikoerper, d2r2 antikoerper, Drd-2 antikoerper, d2dr antikoerper, d2r antikoerper, drd2 antikoerper, drd2-B antikoerper, DRD2 antikoerper, D2 antikoerper, CG18741 antikoerper, CG7569 antikoerper, DAMB antikoerper, DDR2 antikoerper, DOPR2 antikoerper, DOPR99B antikoerper, Dar2 antikoerper, Dmel\\CG18741 antikoerper, DopD1 antikoerper, DopR99B antikoerper, DopRII antikoerper, dopamine receptor D2 antikoerper, dopamine receptor D2 like antikoerper, dopamine receptor D2 L homeolog antikoerper, dopamine receptor D2 S homeolog antikoerper, Dopamine 1-like receptor 2 antikoerper, DRD2 antikoerper, drd2l antikoerper, drd2 antikoerper, Drd2 antikoerper, drd2.L antikoerper, drd2.S antikoerper, Dop1R2 antikoerper

Hintergrund: Name/Gene ID: DRD2
Subfamily: Dopamine
Family: GPCR

Synonyms: DRD2, D2 dopamine receptor, D2R, Dopamine receptor D2 isoform, Dopamine 2 receptor, D(2) dopamine receptor, D2DR, Dopamine D2 receptor, Dopamine receptor D2

Gen-ID: 1813

Pathways: Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Inositol Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Feeding Behaviour, Negative Regulation of Transporter Activity, Regulation of long-term Neuronal Synaptic Plasticity