Adenosine A2b Receptor Antikörper (Internal Region)
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- Target Alle Adenosine A2b Receptor (ADORA2B) Antikörper anzeigen
- Adenosine A2b Receptor (ADORA2B)
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Bindungsspezifität
- Internal Region
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Reaktivität
- Human
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser Adenosine A2b Receptor Antikörper ist unkonjugiert
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Applikation
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Marke
- IHC-plus™
- Spezifität
- Human Adenosine A2b Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- Homologie
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon (100%) Monkey, Marmoset, Horse (94%) Rabbit (88%) Dog (81%).
- Aufreinigung
- Immunoaffinity purified
- Immunogen
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Synthetic 16 amino acid peptide from internal region of human Adenosine A2b Receptor. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon (100%), Monkey, Marmoset, Horse (94%), Rabbit (88%), Dog (81%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product ADORA2B Primärantikörper
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- Applikationshinweise
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Approved: IHC, IHC-P (10 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after proteinase K antigen retrieval. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. - Kommentare
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Target Species of Antibody: Human
- Testdurchführung
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- Lot specific
- Buffer
- PBS, less than 0.1 % sodium azide.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- Haltbarkeit
- 12 months
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- Target
- Adenosine A2b Receptor (ADORA2B)
- Andere Bezeichnung
- ADORA2B/Adenosine A2B Receptor (ADORA2B Produkte)
- Synonyme
- si:dkey-13b16.1 antikoerper, ADORA2B antikoerper, LOC606745 antikoerper, LOC100220469 antikoerper, ADORA2 antikoerper, A2BAR antikoerper, A2BR antikoerper, A2b antikoerper, AA2BR antikoerper, AI480866 antikoerper, AI605384 antikoerper, adenosine A2b receptor antikoerper, adenosine A2B receptor antikoerper, adora2b antikoerper, ADORA2B antikoerper, Adora2b antikoerper
- Hintergrund
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Name/Gene ID: ADORA2B
Subfamily: Adenosine
Family: GPCR
Synonyms: ADORA2B, ADORA2, A2b receptor, Adenosine A2b receptor, Adenosine a2b, Adenosine receptor A2b, A2b adenosine receptor - Gen-ID
- 136
- Pathways
- cAMP Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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