Ziege anti-Kaninchen IgG (Heavy & Light Chain) Antikörper (Alkaline Phosphatase (AP)) - Preadsorbed
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- Target Alle IgG Produkte
- IgG
- Fragment
- F(ab')2 fragment
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Bindungsspezifität
- Heavy & Light Chain
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Reaktivität
- Kaninchen
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Wirt
- Ziege
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Klonalität
- Polyklonal
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Konjugat
- Alkaline Phosphatase (AP)
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Applikation
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Spezifität
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Rabbit IgG and Rabbit Serum.
- Aufreinigung
- Preadsorption: immunoaffinity chromatography using Rabbit IgG coupled to agarose beads
- Sterilität
- Sterile filtered
- Immunogen
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Immunogen: Rabbit IgG whole molecule
- Isotyp
- IgG
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- Applikationshinweise
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Immunohistochemistry Dilution: 1:200-1:1,000
Application Note: This product has been assayed against 1.0 μg of Rabbit IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:2,000-1:10,000
Western Blot Dilution: 1:500-1:2,500
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Rekonstitution
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Reconstitution Buffer: Restore with deionized water (or equivalent)
- Konzentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
- Handhabung
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Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed.
Centrifuge product if not completely clear after standing at room temperature. - Lagerung
- 4 °C
- Informationen zur Lagerung
- Store vial at 4 °C prior to restoration. This product is stable for several weeks at 4 °C as an undiluted liquid.
- Haltbarkeit
- 12 months
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- Target
- IgG
- Abstract
- IgG Produkte
- Substanzklasse
- Antibody
- Hintergrund
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Synonyms: Goat F(ab')2 Anti-Rabbit IgG Alkaline Phosphatase Conjugated Antibody, Goat Fab2 Anti-Rabbit IgG Antibody alk phos Conjugation
Background: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
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