Kaninchen anti-Ziege IgG (Heavy & Light Chain) Antikörper (Atto 550)

Details zu Produkt Nr. ABIN1043945, Anbieter: Anmelden zum Anzeigen
Antigen
Epitop
Heavy & Light Chain
6508
2071
2051
1189
814
645
311
269
33
30
30
15
12
12
12
12
11
7
3
2
1
1
1
1
1
1
1
Reaktivität
Ziege
3172
3055
2511
1746
1579
820
766
504
483
430
411
409
335
291
277
173
159
128
54
30
30
26
15
12
12
8
7
5
5
4
4
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Wirt
Kaninchen
7495
5380
2124
1082
552
357
89
73
55
35
23
15
8
5
1
1
1
Klonalität
Polyklonal
Konjugat
Atto 550
2298
1890
1829
1326
792
657
536
340
269
174
147
145
143
140
119
112
112
102
98
94
93
91
89
86
84
81
79
78
75
73
68
68
65
61
55
54
54
54
54
53
53
53
53
53
53
52
49
48
43
41
38
36
32
31
23
23
22
20
19
17
16
15
15
14
14
14
12
12
12
12
11
11
11
10
10
10
10
9
9
9
9
9
9
8
8
8
8
8
7
6
6
6
6
6
6
6
5
5
5
5
5
5
5
5
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Applikation
FLISA, Fluorescence Microscopy (FM), Western Blotting (WB)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen

Immunogen: Goat IgG whole molecule

Isotyp IgG
Spezifität Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Goat IgG and Goat Serum.
Kreuzreaktivität (Details) Pre-Adsorbed
Produktmerkmale Anti-Goat IgG (H&L) conjugated by ATTO 550 is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
This product is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Reinigung Goat IgG (H&L) conjugated by ATTO 550 was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Goat IgG and Goat Serum.  No reaction was observed against Human, Mouse or Rabbit Serum Proteins.  This antibody will react with heavy chains of Goat IgG and with light chains of most Goat immunoglobulins.
Labeling Ratio 1.9
Antigen
Hintergrund

Synonyms: rabbit anti-Goat IgG ATTO 550 Conjugated Antibody, rabbit anti-Goat IgG Antibody ATTO550 Conjugation

Background: Anti-Goat IgG ATTO dye Antibody generated in rabbit detects goat IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.

Applikations-hinweise

Application Note: Anti-Goat IgG (H&L) conjugated by ATTO 550 is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

FLISA Dilution: >1:20,000

Western Blot Dilution: >1:10,000

IF Microscopy Dilution: >1:5,000

Kommentare

The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution

Reconstitution Volume: 500 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration 1.0 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid cycles of freezing and thawing.
Product is photosensitive and should be protected from light.
Lagerung RT,4 °C,-20 °C
Informationen zur Lagerung Store vial at -20 °C or below prior to opening. Store the vial at -20 °C or below after dilution.
Haltbarkeit 12 months
Bilder des Herstellers
 image for Rabbit anti-Goat IgG (Heavy & Light Chain) antibody (Atto 550) (ABIN1043945) Rabbit anti-Goat IgG (Heavy & Light Chain) antibody (Atto 550)
Western Blotting (WB) image for Rabbit anti-Goat IgG (Heavy & Light Chain) antibody (Atto 550) (ABIN1043945) Western Blot of Atto 550 conjugated Rabbit anti-Goat IgG antibody. Lane 1: Goat IgG. ...
 image for Rabbit anti-Goat IgG (Heavy & Light Chain) antibody (Atto 550) (ABIN1043945) Rabbit anti-Goat IgG (Heavy & Light Chain) antibody (Atto 550) (Image 3)