SLC12A4 Antikörper (AA 932-1043)

Produktnummer ABIN1043014

Produktdetails anti-SLC12A4 Antikörper

Target: SLC12A4 (Solute Carrier Family 12 (Potassium-Chloride Transporter) Member 4 (SLC12A4))

Bindungsspezifität: AA 932-1043

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SLC12A4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (fixed cells) (IF/ICC), Immunoprecipitation (IP)

Verwendungszweck: Anti-K+/Cl-Cotransporter (KCC2) Mouse Monoclonal Antibody

Spezifität: This antibody recognizes human, mouse and rat KCC2.

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Purified by Protein G affinity chromatography.

Immunogen: Fusion protein consisting of aa 932-1043 of rat KCC2 (accession no. NP_599190).

Klon: S1-12

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Immunoblotting: use at 3 μg/mL. A band of ~140 kDa is detected.
Immunohistochemistry: use at 1-5 μg/mL.
Positive control: Adult rat brain
These are recommended concentrations,
Enduser should determine optimal concentrations for their applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Dilute in PBS or medium which is identical to that used in the assay system.

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 50 % glycerol, 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: This antibody is stable for at least one (1) year at -20°C.

Details zu SLC12A4

Target: SLC12A4 (Solute Carrier Family 12 (Potassium-Chloride Transporter) Member 4 (SLC12A4))

Andere Bezeichnung: K+/Cl- Cotransporter (SLC12A4 Produkte)

Synonyme: KCC1 antikoerper, kcc1 antikoerper, SLC12A4 antikoerper, AW546649 antikoerper, RBCKCC1 antikoerper, Kcc1 antikoerper, solute carrier family 12 member 4 antikoerper, solute carrier family 12 (potassium/chloride transporter), member 4 L homeolog antikoerper, solute carrier family 12, member 4 antikoerper, SLC12A4 antikoerper, slc12a4.L antikoerper, Slc12a4 antikoerper

Hintergrund: Solute carrier family 12 member 5,Mediates electroneutral potassium-chloride cotransport in mature neurons and is required for neuronal Cl(-) homeostasis. As major extruder of intracellular chloride, it establishes the low neuronal Cl(-) levels required for chloride influx after binding of GABA-A and glycine to their receptors, with subsequent hyperpolarization and neuronal inhibition (PubMed:9930699). Involved in the regulation of dendritic spine formation and maturation (PubMed:22345354). {PubMed:22345354, PubMed:9930699}.,KCC2 is a member of the cation-chloride cotransporter gene family. KCCs lower intracellular chloride concentrations below the electrochemical equilibrium potential. Depending on the concentration gradients of potassium and chloride, KCC2 can operate as a net efflux or influx pathway. KCC2 is expressed at high levels in neurons throughout the nervous system and is localized at inhibitory synapses of the spinal cord. Studies in mice have shown that KCC2 reduces GABA's inhibitory signaling resulting in motor defects, epilepsy, and anxiety-like behavior.,Membrane, Multi-pass membrane protein.,Electroneutral potassium-chloride cotransporter 2, Furosemide-sensitive K-Cl cotransporter, K-Cl cotransporter 2, rKCC2, Neuronal K-Cl cotransporter

NCBI Accession: NP_599190

UniProt: Q63633