Rec.Pr.Thyrosin Phosphate (RPTP) Antikörper
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- Target
- Rec.Pr.Thyrosin Phosphate (RPTP)
- Reaktivität
- Human, Schwein, Ratte
- Wirt
- Maus
- Klonalität
- Monoklonal
- Applikation
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
- Spezifität
- Monoclonal antibodies 3G4 and 3D7 have been directed against the extracellular domain of RPTPµ.
- Produktmerkmale
- Cell adhesion
- Aufreinigung
- Purified
- Klon
- 3D7
- Isotyp
- IgG2a
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- Applikationshinweise
- 3D7 is useful for western blot, immunofluorescence, immunoprecipitation and flow cytometry. Optimal antibody dilution should be determined by titration.
- Kommentare
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3D7 is a mouse monoclonal IgG2a antibody derived by fusion of SP2/0 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with ExG protein, secreted by NIH-3T3 transfected cells and purified with glutahione beads. The cells have been transfected using a fusion construct, termed ExG, encoding the entire ectodomain of the human RPTPµ molecule (aa 1-742), fused COOH terminally to GST.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Buffer
- PBS containing 0.09% sodium azide.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C
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Receptor protein-tyrosine phosphatase RPTPmu binds to and dephosphorylates the catenin p120(ctn)." in: The Journal of biological chemistry, Vol. 275, Issue 15, pp. 11264-9, (2000) (PubMed).
: "
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Receptor protein-tyrosine phosphatase RPTPmu binds to and dephosphorylates the catenin p120(ctn)." in: The Journal of biological chemistry, Vol. 275, Issue 15, pp. 11264-9, (2000) (PubMed).
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- Target
- Rec.Pr.Thyrosin Phosphate (RPTP)
- Hintergrund
- Receptor-like protein tyrosine phosphatases (RPTPs) represent a new family of transmembrane proteins that are thought to transduce external signals by dephosphorylating phosphotyrosine residues on intracellular substrates. RPTPµ is a prototypic receptor-like protein-tyrosine phosphatase (RPTP) that mediates homotypic cell-cell interactions. Intracellularly, RPTPµ consists of a relatively large juxtamembrane region (about 150 amino acids) and two conserved phosphatase domains. As in most RPTPs, the first (membrane-proximal) PTP domain of RPTPµ is catalytically active, whereas its second (C-terminal) domain is inactive. RPTPµ is involved in cell-cell adhesion through homophilic interactions and may also play a key role in signal transduction and growth control.
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