Kaninchen anti-Schwein IgM (Heavy Chain) Antikörper
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- Target Alle IgM Produkte
- IgM
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Bindungsspezifität
- Heavy Chain
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Reaktivität
- Schwein
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Applikation
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Spezifität
- IgM µ chain
- Produktmerkmale
- Concentration Definition: by Refractometry
- Aufreinigung
- This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, Swine IgM and Swine Serum. No reaction was observed against Swine IgG.
- Immunogen
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Immunogen: Swine IgM mu heavy chain
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- Applikationshinweise
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Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: Anti-Swine IgM antibody are ideal for ELISA, western blotting, Immunohistochemistry, as well as other antibody detection methods.
ELISA Dilution: 1:20,000 - 1:100,000
Western Blot Dilution: 1:2,000 - 1:10,000
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
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Reconstitution Volume: 2.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Konzentration
- 75 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- RT,4 °C,-20 °C
- Haltbarkeit
- 12 months
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- Target
- IgM
- Abstract
- IgM Produkte
- Substanzklasse
- Antibody
- Hintergrund
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Synonyms: rabbit anti-Swine IgM (mu) Antibody, rabbit anti Swine IgM mu
Background: Anti-Swine IgM antibody generated in rabbit specifically detects swine IgM. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Swine IgM antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.
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