Ziege anti-Ratte (Rattus) IgG (Heavy & Light Chain) Antikörper (Biotin)

Details zu Produkt Nr. ABIN102119, Anbieter: Anmelden zum Anzeigen
Antigen
Epitop
Heavy & Light Chain
6294
1955
1755
1065
889
727
375
300
35
34
31
15
11
7
3
3
1
1
1
1
1
1
1
Reaktivität
Ratte (Rattus)
3105
2903
2388
1642
1483
784
713
472
465
401
370
362
313
294
279
146
128
113
42
34
29
26
16
15
11
10
8
7
5
5
4
4
3
3
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
Wirt
Ziege
7018
5159
1943
1091
523
358
89
84
55
35
23
15
8
5
3
1
1
Klonalität
Polyklonal
Konjugat
Biotin
2220
1832
1749
1253
763
687
443
342
196
155
155
150
123
122
122
102
100
96
94
89
85
84
78
77
75
73
68
68
68
65
61
55
55
54
54
54
53
53
53
53
53
53
48
48
47
41
38
35
35
34
32
31
24
23
23
22
20
18
18
17
16
15
15
15
14
14
14
12
12
12
12
12
11
10
10
10
10
10
9
9
9
9
8
8
8
8
8
7
6
6
6
6
6
6
6
5
5
5
5
5
5
5
5
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Applikation
ELISA
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Rat IgG whole molecule
Isotyp IgG
Spezifität IgG (H&L)
Produktmerkmale RAT IgG Biotin Conjugated antibody detects rat immunoglobulin G.
Concentration Definition: by UV absorbance at 280 nm
Antigen
Hintergrund Immunoglobuoin G is a molecule of about 150 kDa composed of four peptide chains. Each IgG contains two identical γ heavy chains of about 51 kDa and two identical light chains of about 26 kDa, thus a tetrameric quaternary structure. The two heavy chains are linked to each other and to a light chain each by disulfide bonds. The resulting tetramer has two identical halves, which together form the Y-like shape. Each end of the fork contains an identical antigen binding site. The Fc regions of IgGs bear a highly conserved N-glycosylation site.
Molekulargewicht 150 kDa
Applikations-hinweise Anti-RAT IgG Biotin Conjugated antibody is suitable for immunoassays where specificity to the Rat immunoglobulin heavy and or light chain regions is desired. Anti-Rat antibody has been assayed against 1.0 µg of Rat IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:100,000 to 1:500,000 is suggested for this product. Optimal concentrations in immunoassays should be determined by the researcher.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution Restore with deionized water (or equivalent)
Konzentration 2.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handhabung Aliquot to Avoid repeated freezing and thawing.
Bilder des Herstellers
Western Blotting (WB) image for Goat anti-Rat (Rattus) IgG (Heavy & Light Chain) antibody (Biotin) (ABIN102119) Western Blot of Anti-Rat IgG (H&L) (GOAT) Antibody . Lane M: 3 μl Molecular Ladder. L...
Western Blotting (WB) image for Goat anti-Rat (Rattus) IgG (Heavy & Light Chain) antibody (Biotin) (ABIN102119) Western Blot of Goat anti-Rat IgG (H&L) Antibody Biotin Conjugated. Lane 1: Rat IgG. ...
Allgemeine Veröffentlichungen Bayer, Wilchek: "Protein biotinylation." in: Methods in enzymology, Vol. 184, pp. 138-60, 1990 (PubMed).