Ziege anti-Ratte (Rattus) IgG (Heavy & Light Chain) Antikörper (Biotin)

Details zu Produkt Nr. ABIN102119, Anbieter: Anmelden zum Anzeigen
Antigen
Epitop
Heavy & Light Chain
6316
1976
1804
1067
1034
856
375
303
35
34
31
15
11
7
3
3
2
1
1
1
1
1
1
1
Reaktivität
Ratte (Rattus)
3126
2957
2440
1679
1523
808
698
466
459
402
376
365
307
290
284
148
138
121
43
34
29
26
16
15
11
10
8
7
5
5
4
4
4
3
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
Wirt
Ziege
7121
5171
1965
1090
594
377
91
84
55
35
23
15
8
5
3
1
1
Klonalität
Polyklonal
Konjugat
Biotin
2243
1830
1779
1291
766
690
455
320
215
171
161
155
155
141
137
127
110
107
102
94
89
84
78
77
75
73
71
68
65
64
60
58
55
54
54
54
53
53
53
53
53
53
52
48
41
38
37
35
31
24
23
23
22
20
18
18
18
17
16
15
15
15
14
14
14
14
14
12
12
12
12
12
11
10
10
10
10
10
9
9
9
9
8
8
8
8
8
8
7
6
6
6
6
6
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Applikation
ELISA
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Rat IgG whole molecule
Isotyp IgG
Spezifität IgG (H&L)
Produktmerkmale RAT IgG Biotin Conjugated antibody detects rat immunoglobulin G.
Concentration Definition: by UV absorbance at 280 nm
Antigen IgG
Hintergrund Immunoglobuoin G is a molecule of about 150 kDa composed of four peptide chains. Each IgG contains two identical γ heavy chains of about 51 kDa and two identical light chains of about 26 kDa, thus a tetrameric quaternary structure. The two heavy chains are linked to each other and to a light chain each by disulfide bonds. The resulting tetramer has two identical halves, which together form the Y-like shape. Each end of the fork contains an identical antigen binding site. The Fc regions of IgGs bear a highly conserved N-glycosylation site.
Molekulargewicht 150 kDa
Applikations-hinweise Anti-RAT IgG Biotin Conjugated antibody is suitable for immunoassays where specificity to the Rat immunoglobulin heavy and or light chain regions is desired. Anti-Rat antibody has been assayed against 1.0 µg of Rat IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:100,000 to 1:500,000 is suggested for this product. Optimal concentrations in immunoassays should be determined by the researcher.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution Restore with deionized water (or equivalent)
Konzentration 2.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handhabung Aliquot to Avoid repeated freezing and thawing.
Bilder des Herstellers
Western Blotting (WB) image for Goat anti-Rat (Rattus) IgG (Heavy & Light Chain) antibody (Biotin) (ABIN102119) Western Blot of Goat anti-Rat IgG (H&L) Antibody Biotin Conjugated. Lane 1: Rat IgG. ...
Western Blotting (WB) image for Goat anti-Rat (Rattus) IgG (Heavy & Light Chain) antibody (Biotin) (ABIN102119) Western Blot of Anti-Rat IgG (H&L) (GOAT) Antibody . Lane M: 3 μl Molecular Ladder. L...
Western Blotting (WB) image for Goat anti-Rat (Rattus) IgG (Heavy & Light Chain) antibody (Biotin) (ABIN102119) Western Blot of Goat anti-Rat IgG (H&L) Antibody Biotin Conjugated. Lane 1: Rat IgG. ...
Allgemeine Veröffentlichungen Bayer, Wilchek: "Protein biotinylation." in: Methods in enzymology, Vol. 184, pp. 138-60, 1990 (PubMed).