Ziege anti-Ratte (Rattus) IgG (Heavy & Light Chain) Antikörper (Biotin)

Details zu Produkt Nr. ABIN102119, Anbieter: Anmelden zum Anzeigen
Antigen
Epitop
Heavy & Light Chain
5080
1879
1842
1048
1021
847
326
309
35
34
31
12
8
7
3
3
2
1
1
1
1
1
1
1
Reaktivität
Ratte (Rattus)
2879
2595
2171
1545
1387
751
599
453
426
389
366
355
295
278
265
145
131
113
41
30
29
26
16
11
10
8
7
6
5
5
4
4
4
3
3
3
3
3
3
2
2
2
1
1
1
1
1
Wirt
Ziege
6462
4916
1656
986
519
356
93
67
51
18
8
5
5
5
2
1
1
Klonalität
Polyklonal
Konjugat
Biotin
2210
1816
1734
1282
772
695
446
300
219
171
165
141
137
129
110
107
77
75
71
65
65
56
55
54
54
53
53
53
53
53
53
52
50
41
37
35
23
19
18
17
17
15
15
15
14
14
14
14
12
12
11
10
10
10
10
10
9
9
9
9
9
8
8
8
8
6
6
6
6
6
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Applikation
ELISA
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Rat IgG whole molecule
Isotyp IgG
Spezifität IgG (H&L)
Produktmerkmale RAT IgG Biotin Conjugated antibody detects rat immunoglobulin G.
Concentration Definition: by UV absorbance at 280 nm
Hintergrund Immunoglobuoin G is a molecule of about 150 kDa composed of four peptide chains. Each IgG contains two identical γ heavy chains of about 51 kDa and two identical light chains of about 26 kDa, thus a tetrameric quaternary structure. The two heavy chains are linked to each other and to a light chain each by disulfide bonds. The resulting tetramer has two identical halves, which together form the Y-like shape. Each end of the fork contains an identical antigen binding site. The Fc regions of IgGs bear a highly conserved N-glycosylation site.
Molekulargewicht 150 kDa
Applikations-hinweise Anti-RAT IgG Biotin Conjugated antibody is suitable for immunoassays where specificity to the Rat immunoglobulin heavy and or light chain regions is desired. Anti-Rat antibody has been assayed against 1.0 µg of Rat IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:100,000 to 1:500,000 is suggested for this product. Optimal concentrations in immunoassays should be determined by the researcher.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution Restore with deionized water (or equivalent)
Konzentration 2.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handhabung Aliquot to Avoid repeated freezing and thawing.
Bilder des Herstellers
Western Blotting (WB) image for Goat anti-Rat (Rattus) IgG (Heavy & Light Chain) antibody (Biotin) (ABIN102119) Western Blot of Goat anti-Rat IgG (H&L) Antibody Biotin Conjugated. Lane 1: Rat IgG. ...
Western Blotting (WB) image for Goat anti-Rat (Rattus) IgG (Heavy & Light Chain) antibody (Biotin) (ABIN102119) Western Blot of Anti-Rat IgG (H&L) (GOAT) Antibody . Lane M: 3 μl Molecular Ladder. L...
Western Blotting (WB) image for Goat anti-Rat (Rattus) IgG (Heavy & Light Chain) antibody (Biotin) (ABIN102119) Western Blot of Goat anti-Rat IgG (H&L) Antibody Biotin Conjugated. Lane 1: Rat IgG. ...
Allgemeine Veröffentlichungen Bayer, Wilchek: "Protein biotinylation." in: Methods in enzymology, Vol. 184, pp. 138-60, 1990 (PubMed).