Esel anti-Kaninchen IgG (Heavy & Light Chain) Antikörper (HRP)

Details zu Produkt Nr. ABIN101955, Anbieter: Anmelden zum Anzeigen
Antigen
Epitop
Heavy & Light Chain
6301
1957
1804
1065
889
727
375
301
35
34
31
15
11
7
3
3
1
1
1
1
1
1
1
Reaktivität
Kaninchen
3073
2887
2376
1634
1481
785
690
459
456
397
369
364
301
288
280
146
128
114
42
34
29
26
16
15
11
10
8
7
5
5
4
4
3
3
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
Wirt
Esel
6987
5101
1926
1048
535
361
90
84
55
35
23
15
8
5
3
1
1
Klonalität
Polyklonal
Konjugat
HRP
2224
1809
1765
1261
763
687
455
310
196
155
155
150
127
122
122
102
102
96
94
89
84
79
78
75
73
68
68
68
65
61
55
55
54
54
54
53
53
53
53
53
53
53
48
48
41
38
35
34
31
24
23
23
22
20
18
18
17
16
15
15
15
14
14
14
14
14
12
12
12
12
12
10
10
10
10
10
9
9
9
9
8
8
8
8
8
7
6
6
6
6
6
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Applikation
Dot Blot (DB), Immunoelectron Microscopy (IEM), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Anti-Rabbit IgG was produced by repeated immunization with rabbit whole IgG molecule in donkey.
Isotyp IgG
Spezifität IgG (H&L)
Produktmerkmale Anti-Rabbit IgG peroxidase conjugated antibody generated in donkey detects specifically rabbit IgG. This secondary peroxidase conjugated antibody anti-Rabbit is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Concentration Definition: by UV absorbance at 280 nm
Reinigung This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Donkey Serum, Rabbit IgG and Rabbit Serum.
Antigen
Hintergrund Synonyms: Rabbit Antibody peroxidase conjugated, Dk-a-Rabbit peroxidase conjugated IgG, Rabbit proxidase conjugated Antibody in donkey, Rabbit Secondary peroxidase conjugated Antibody, peroxidase.
Applikations-hinweise Anti-Rabbit IgG peroxidase conjugated antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution Restore with deionized water (or equivalent)
Konzentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Konservierungs-mittel Gentamicin sulfate
Handhabung Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Bilder des Herstellers
Western Blotting (WB) image for Donkey anti-Rabbit IgG (Heavy & Light Chain) antibody (HRP) (ABIN101955) Western Blot of Anti-Rabbit IgG (H&L) (DONKEY) Antibody (Min X Bv Ch Gt GP Ham Hs Hu ...
Western Blotting (WB) image for Donkey anti-Rabbit IgG (Heavy & Light Chain) antibody (HRP) (ABIN101955) Western Blot of Peroxidase conjugated Donkey anti-Rabbit IgG antibody. Lane 1: Rabbit...
Allgemeine Veröffentlichungen Farr, Nakane: "Immunohistochemistry with enzyme labeled antibodies: a brief review." in: Journal of immunological methods, Vol. 47, Issue 2, pp. 129-44, 1982 (PubMed).