Esel anti-Kaninchen IgG (Heavy & Light Chain) Antikörper (HRP)

Details zu Produkt Nr. ABIN101955, Anbieter: Anmelden zum Anzeigen
Antigen
Epitop
Heavy & Light Chain
6367
2017
1755
1137
784
649
374
303
35
34
31
15
11
7
3
3
1
1
1
1
1
1
1
Reaktivität
Kaninchen
3054
2855
2343
1624
1463
773
740
499
484
423
403
401
311
295
274
159
151
111
49
34
30
26
16
15
11
10
8
7
5
5
4
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Wirt
Esel
7023
5236
1914
1077
508
358
89
84
52
35
23
15
8
5
3
1
1
Klonalität
Polyklonal
Konjugat
HRP
2219
1844
1762
1274
794
719
442
342
187
145
140
140
116
112
102
95
94
92
91
89
84
84
78
75
73
72
68
68
67
65
61
55
54
54
54
54
53
53
53
53
53
53
48
48
46
41
38
35
34
34
32
31
24
23
23
22
20
18
18
17
16
15
15
15
14
14
14
12
12
12
12
12
12
10
10
10
10
10
9
9
9
9
8
8
8
8
8
7
6
6
6
6
6
6
6
5
5
5
5
5
5
5
5
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Applikation
Dot Blot (DB), Immunoelectron Microscopy (IEM), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Anti-Rabbit IgG was produced by repeated immunization with rabbit whole IgG molecule in donkey.
Isotyp IgG
Spezifität IgG (H&L)
Produktmerkmale Anti-Rabbit IgG peroxidase conjugated antibody generated in donkey detects specifically rabbit IgG. This secondary peroxidase conjugated antibody anti-Rabbit is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Concentration Definition: by UV absorbance at 280 nm
Reinigung This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Donkey Serum, Rabbit IgG and Rabbit Serum.
Antigen
Hintergrund Synonyms: Rabbit Antibody peroxidase conjugated, Dk-a-Rabbit peroxidase conjugated IgG, Rabbit proxidase conjugated Antibody in donkey, Rabbit Secondary peroxidase conjugated Antibody, peroxidase.
Applikations-hinweise Anti-Rabbit IgG peroxidase conjugated antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution Restore with deionized water (or equivalent)
Konzentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Konservierungs-mittel Gentamicin sulfate
Handhabung Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Bilder des Herstellers
Western Blotting (WB) image for Donkey anti-Rabbit IgG (Heavy & Light Chain) antibody (HRP) (ABIN101955) Western Blot of Anti-Rabbit IgG (H&L) (DONKEY) Antibody (Min X Bv Ch Gt GP Ham Hs Hu ...
Western Blotting (WB) image for Donkey anti-Rabbit IgG (Heavy & Light Chain) antibody (HRP) (ABIN101955) Western Blot of Peroxidase conjugated Donkey anti-Rabbit IgG antibody. Lane 1: Rabbit...
Allgemeine Veröffentlichungen Farr, Nakane: "Immunohistochemistry with enzyme labeled antibodies: a brief review." in: Journal of immunological methods, Vol. 47, Issue 2, pp. 129-44, 1982 (PubMed).