Esel anti-Kaninchen IgG (Heavy & Light Chain) Antikörper (HRP)

Details zu Produkt Nr. ABIN101955, Anbieter: Anmelden zum Anzeigen
Antigen
Epitop
Heavy & Light Chain
6009
1873
1825
1047
1014
856
334
304
35
34
31
15
11
7
3
3
2
1
1
1
1
1
1
1
Reaktivität
Kaninchen
3050
2888
2388
1635
1490
789
666
448
444
384
356
352
294
280
265
145
130
116
41
30
29
26
16
11
10
8
7
6
5
5
4
4
4
3
3
3
3
3
3
2
2
2
1
1
1
1
1
Wirt
Esel
6920
5014
1922
1075
574
352
89
67
54
35
23
12
8
5
3
1
1
Klonalität
Polyklonal
Konjugat
HRP
2216
1806
1755
1290
766
690
445
321
217
171
160
141
137
127
110
107
102
94
89
84
78
77
75
73
71
68
65
64
60
55
55
54
54
54
53
53
53
53
53
53
52
48
41
38
37
35
31
24
23
23
22
20
18
18
17
16
15
15
15
15
14
14
14
14
14
12
12
12
12
12
11
10
10
10
10
10
9
9
9
9
8
8
8
8
8
8
7
6
6
6
6
6
6
6
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Applikation
Dot Blot (DB), Immunoelectron Microscopy (IEM), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Immunogen Anti-Rabbit IgG was produced by repeated immunization with rabbit whole IgG molecule in donkey.
Isotyp IgG
Spezifität IgG (H&L)
Produktmerkmale Anti-Rabbit IgG peroxidase conjugated antibody generated in donkey detects specifically rabbit IgG. This secondary peroxidase conjugated antibody anti-Rabbit is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Concentration Definition: by UV absorbance at 280 nm
Reinigung This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Donkey Serum, Rabbit IgG and Rabbit Serum.
Hintergrund Synonyms: Rabbit Antibody peroxidase conjugated, Dk-a-Rabbit peroxidase conjugated IgG, Rabbit proxidase conjugated Antibody in donkey, Rabbit Secondary peroxidase conjugated Antibody, peroxidase.
Applikations-hinweise Anti-Rabbit IgG peroxidase conjugated antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution Restore with deionized water (or equivalent)
Konzentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Konservierungs-mittel Gentamicin sulfate
Handhabung Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Bilder des Herstellers
Western Blotting (WB) image for Donkey anti-Rabbit IgG (Heavy & Light Chain) antibody (HRP) (ABIN101955) Western Blot of Peroxidase conjugated Donkey anti-Rabbit IgG antibody. Lane 1: Rabbit...
Western Blotting (WB) image for Donkey anti-Rabbit IgG (Heavy & Light Chain) antibody (HRP) (ABIN101955) Western Blot of Anti-Rabbit IgG (H&L) (DONKEY) Antibody (Min X Bv Ch Gt GP Ham Hs Hu ...
Western Blotting (WB) image for Donkey anti-Rabbit IgG (Heavy & Light Chain) antibody (HRP) (ABIN101955) Western Blot of Peroxidase conjugated Donkey anti-Rabbit IgG antibody. Lane 1: Rabbit...
Allgemeine Veröffentlichungen Farr, Nakane: "Immunohistochemistry with enzyme labeled antibodies: a brief review." in: Journal of immunological methods, Vol. 47, Issue 2, pp. 129-44, 1982 (PubMed).