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Ziege anti-Esel IgG (Fc Region) Antikörper

ELISA, IHC, WB Polyclonal IgG unconjugated
Produktnummer ABIN101145
  • Target Alle IgG Produkte
    IgG
    Bindungsspezifität
    • 2272
    • 456
    • 363
    • 188
    • 130
    • 123
    • 92
    • 29
    • 28
    • 28
    • 17
    • 9
    • 6
    • 6
    • 6
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    Fc Region
    Reaktivität
    • 688
    • 684
    • 569
    • 442
    • 378
    • 235
    • 199
    • 156
    • 138
    • 130
    • 113
    • 97
    • 95
    • 93
    • 84
    • 57
    • 45
    • 33
    • 27
    • 16
    • 12
    • 10
    • 8
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Esel
    Wirt
    • 1689
    • 1473
    • 548
    • 246
    • 104
    • 90
    • 33
    • 30
    • 21
    • 16
    • 15
    • 11
    • 11
    • 1
    Ziege
    Klonalität
    • 3433
    • 199
    • 1
    Polyklonal
    Applikation
    • 1780
    • 1713
    • 1689
    • 1442
    • 934
    • 910
    • 883
    • 463
    • 243
    • 243
    • 238
    • 100
    • 92
    • 85
    • 76
    • 50
    • 50
    • 32
    • 31
    • 27
    • 24
    • 22
    • 21
    • 16
    • 11
    • 8
    • 5
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Spezifität
    IgG F(c)
    Kreuzreaktivität
    Esel
    Produktmerkmale
    Concentration Definition: by UV absorbance at 280 nm
    Aufreinigung
    This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Donkey IgG, Donkey IgG F(c) and Donkey Serum.  No reaction was observed against Donkey IgG F(ab).
    Immunogen

    Immunogen: Donkey IgG F(c) fragment

    Isotyp
    IgG
  • Applikationshinweise

    Immunohistochemistry Dilution: 1:1,000 - 1:5,000

    Application Note: Anti-Donkey IgG F(c) antibody is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

    ELISA Dilution: 1:20,000 - 1:100,000

    Western Blot Dilution: 1:2,000 - 1:10,000

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution

    Reconstitution Volume: 5.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Konzentration
    10.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    RT,4 °C,-20 °C
    Haltbarkeit
    12 months
  • Target
    IgG
    Abstract
    IgG Produkte
    Substanzklasse
    Antibody
    Hintergrund

    Synonyms: goat anti-Donkey IgG F(c) fragment Antibody, goat anti-Donkey IgG Fc fragment Antibody, goat anti Donkey IgG Fc

    Background: Anti-Donkey IgG F(c) generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH . Receptors bind the Fc portion of donkey IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.

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