Kaninchen anti-Huhn IgG (Heavy & Light Chain) Antikörper (Alkaline Phosphatase (AP))
-
- Target Alle IgG Produkte
- IgG
-
Bindungsspezifität
- Heavy & Light Chain
-
Reaktivität
- Huhn
-
Wirt
- Kaninchen
-
Klonalität
- Polyklonal
-
Konjugat
- Alkaline Phosphatase (AP)
-
Applikation
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Spezifität
- IgG (H&L)
- Produktmerkmale
- Concentration Definition: by UV absorbance at 280 nm
- Aufreinigung
- Anti-Chicken IgY / IgG Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgY / IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-Alkaline Phosphatase, and Chicken IgY / IgG. Some light chain cross reactivity may be observed against chicken immunoglobulins.
- Sterilität
- Sterile filtered
- Immunogen
-
Immunogen: Chicken IgY / IgG whole molecule
- Isotyp
- IgG
-
-
- Applikationshinweise
-
Immunohistochemistry Dilution: 1:200 - 1:1,000
Application Note: Anti-Chicken IgG Alk Phos conjugate is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.
ELISA Dilution: 1:2,000 - 1:10,000
Western Blot Dilution: 1:500 - 1:2,500
- Kommentare
-
Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Format
- Liquid
- Konzentration
- 1.0 mg/mL
- Buffer
-
Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.1 % (w/v) Sodium Azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
-
Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed. - Lagerung
- 4 °C
- Haltbarkeit
- 12 months
-
- Target
- IgG
- Abstract
- IgG Produkte
- Substanzklasse
- Antibody
- Hintergrund
-
Synonyms: Rabbit anti-Chicken IgG Alkaline Phosphatase Conjugated Antibody, Rabbit anti-Chicken IgG Antibody Alk Phos Conjugation, Chicken Secondary Antibody
Background: Anti-Chicken IgG Alkaline Phosphatase Antibody generated in rabbit detects chicken IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
-