anti-Human Phospholipase C beta 1 Antikörper für Immunohistochemistry (Paraffin-embedded Sections)

Recommended Phospholipase C beta 1 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
phospholipase C, beta 1 (phosphoinositide-Specific) (PLCB1) Antikörper
  • PLCB1
  • 3110043I21Rik
  • AI132408
  • Plcb
  • mKIAA0581
  • EIEE12
  • PI-PLC
  • PLC-154
  • PLC-I
  • PLC154
  • PLCB1A
  • PLCB1B
  • PLC'1
  • PLCbeta1
  • Phosphb
  • RATPHOSPHB
  • phospholipase C, beta 1 (phosphoinositide-specific)
  • phospholipase C, beta 1
  • PLCB1
  • plcb1
  • Plcb1
Epitop
C-Term
15
4
3
2
1
1
1
1
1
1
Reaktivität
Human
54
40
38
6
4
4
4
3
2
2
1
1
Wirt
Kaninchen
51
4
1
Klonalität
Polyklonal
Konjugat
Dieser Phospholipase C beta 1 Antikörper ist unkonjugiert
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
34
16
13
10
7
5
3
2
1
1
1
Hersteller
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Bilder

Produktnummer ABIN1688638
268,19 €
Zzgl. Versandkosten 20,00 € und MWSt
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN1385859 IF (p) IHC (p) WB Rabbit IgG Anmelden zum Anzeigen Polyclonal
1 ABIN1688634 IHC (p) Rabbit IgG C-Term Anmelden zum Anzeigen Polyclonal
1 ABIN1415619 IHC (p) WB HRP Rabbit IgG Anmelden zum Anzeigen Polyclonal
1 ABIN1397746 IHC (p) WB Biotin Rabbit IgG Anmelden zum Anzeigen Polyclonal
1 ABIN5552547 IHC (p) Rabbit C-Term Anmelden zum Anzeigen Polyclonal
1 ABIN4346112 IHC IHC (p) Rabbit Anmelden zum Anzeigen Polyclonal
1 ABIN4346113 IHC IHC (p) WB Rabbit Anmelden zum Anzeigen Polyclonal
1 ABIN4346110 IHC IHC (p) Rabbit C-Term Anmelden zum Anzeigen Polyclonal
1 ABIN4346109 IHC IHC (p) Rabbit C-Term Anmelden zum Anzeigen Polyclonal
1 ABIN4346111 IHC IHC (p) Rabbit C-Term Anmelden zum Anzeigen Polyclonal

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Applikation / Reaktivität Human
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Flow Cytometry (FACS) 7 Antikörper
Immunocytochemistry (ICC) 1 Antikörper
Immunofluorescence (IF) 5 Antikörper
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Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antikörper
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General

Antigen phospholipase C, beta 1 (phosphoinositide-Specific) (PLCB1) Antikörper
Epitop C-Term
(15), (4), (3), (2), (1), (1), (1), (1), (1), (1)
Reaktivität Human
(54), (40), (38), (6), (4), (4), (4), (3), (2), (2), (1), (1)
Wirt Kaninchen
(51), (4), (1)
Klonalität
Polyklonal
Konjugat Dieser Phospholipase C beta 1 Antikörper ist unkonjugiert
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(34), (16), (13), (10), (7), (5), (3), (2), (1), (1), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-Phospholipase C beta 1 Antikörper

Target Details Phospholipase C beta 1 Anwendungsinformationen Handhabung Bilder
Produktmerkmale Cellular Localization: Cytoplasm, Membrane
Reinigung Immunogen Affinity Purified
Immunogen Synthetic peptide corresponding to C-terminus of human PLC beta 1.
Isotyp IgG

Target Details Phospholipase C beta 1

Produktdetails anti-Phospholipase C beta 1 Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung PLC beta 1 (PLCB1 Antibody Abstract)
Hintergrund Phosphoinositide-specific phospholipase C (PLC) plays a crucial role in the initiation of receptor mediated signal transduction through the generation of the two second messengers, inositol 1,4,5- triphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. There are many mammalian PLC isozymes, including PLC β1, PLC β2, PLC β3, PLC β4, PLC γ1, PLC γ2, PLC δ1, PLC δ2 and PLC ε, with molecular weights ranging from 85 to 150kDa. PLC β1, one of the PLC β isozymes, exists as two immunologically distinguishable proteins of 150 (PLC-β1a) and 140kDa (PLC β1b). The two isoforms encode in two distinct transcripts and generated by alternative splicing of a single gene. PLC β1a is preferentially expressed in the cytosol, whereas PLC β1b is predominantly localized in the nuclei. PLC β1 is a G-protein-dependent phosphodiesterase that hydrolyses phosphatidylinositol 4,5 biphosphate into inositol 1,4,5-triphosphate and diacylglycerol after the stimulation of a variety of neurotransmitter receptors at the cell surface.
Molekulargewicht 150 kDa
Gen-ID 23236
Pathways WNT Signalweg, AMPK Signaling, Thyroid Hormone Synthesis, Inositol Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, Proton Transport, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, G-protein mediated Events

Anwendungsinformationen

Produktdetails anti-Phospholipase C beta 1 Antikörper Target Details Phospholipase C beta 1 Handhabung Bilder zurück nach oben
Applikationshinweise IHC Dilution: Ready to use, for IHC only
Kommentare

Positive Control:
IHC (p): Brain

Protokoll IHC Procedure: Specimen
Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody.
Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols.
Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:25. The dilutions are estimates, actual results may differ because of variability in methods and protocols.
Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.
Primary Antibody Incubation: Incubate for 30 minutes at room temperature.
Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05 % Tween.
Visualization: Detect the antibody as instructed by the instructions provided with the visualization system.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-Phospholipase C beta 1 Antikörper Target Details Phospholipase C beta 1 Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Buffer pre-diluted immunogen affinity purified rabbit polyclonal antibody in TBS/1 % BSA buffer pH 7.6 with less than 0.1 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen 1. Avoid contact of reagents with eyes and mucous membranes. If reagents come into contact with sensitive areas, wash with copious amounts of water.
2. This product is harmful if swallowed.
3. Consult local or state authorities with regard to recommended method of disposal.
4. Avoid microbial contamination of reagents.
This product contains Sodium AzideTM: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handhabung Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product, therefore, positive and negative controls should be tested simultaneously with unknown specimens.
Lagerung 4 °C

Bilder

Produktdetails anti-Phospholipase C beta 1 Antikörper Target Details Phospholipase C beta 1 Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Phospholipase C beta 1 Antikörper (phospholipase C, beta 1 (phosphoinositide-Specific)) (C-Term) (ABIN1688638) anti-phospholipase C, beta 1 (phosphoinositide-Specific) (PLCB1) (C-Term) antibody