Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

IGF1 Protein (AA 49-118)

IGF1 Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant > 95 % , as determined by Coomassie stained SDS-PAGE. ELISA, FACS Active
Produktnummer ABIN2666830
  • Target Alle IGF1 Proteine anzeigen
    IGF1 (Insulin-Like Growth Factor 1 (IGF1))
    Protein-Typ
    Recombinant
    Biologische Aktivität
    Active
    Proteineigenschaft
    AA 49-118
    Spezies
    • 25
    • 16
    • 7
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Quelle
    • 44
    • 16
    • 8
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Escherichia coli (E. coli)
    Applikation
    ELISA, Flow Cytometry (FACS)
    Reinheit
    > 95 % , as determined by Coomassie stained SDS-PAGE.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau

    Less than 0.1 EU per μg protein as determined by the LAL method.

    Top Product
    Discover our top product IGF1 Protein
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Kommentare

    Biological activity: ED50 = 5 - 20 ng/ml, corresponding to a specific activity of 0.05 - 0.20 x 106 units/mg, as determined by the dose dependent stimulation of MCF-7 cell proliferation.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Rekonstitution
    For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.
    Buffer
    0.22 μm filtered protein solution is in 300 mM ammonium acetate pH 5.5.
    Handhabung
    Avoid repeated freeze/thaw cycles.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.
  • Target
    IGF1 (Insulin-Like Growth Factor 1 (IGF1))
    Andere Bezeichnung
    IGF-I (IGF1 Produkte)
    Synonyme
    IGF-I Protein, IGF1A Protein, IGFI Protein, C730016P09Rik Protein, Igf-1 Protein, Igf-I Protein, Npt2B Protein, IGF-1 Protein, IGFIA Protein, IGF-IB Protein, igf1 Protein, IGF-1L Protein, IGF-1a Protein, IGF1 Protein, igf-1 Protein, igf1-A Protein, igf1.S Protein, xigf1 Protein, insulin like growth factor 1 Protein, insulin-like growth factor 1 Protein, insulin-like growth factor I Protein, insulin like growth factor 1 L homeolog Protein, IGF1 Protein, Igf1 Protein, LOC100136741 Protein, igf1 Protein, LOC678666 Protein, igf1.L Protein, LOC104911603 Protein
    Hintergrund
    IGF-I (insulin-like growth factor 1), initially described in 1957, is a hormone that is important in childhood growth and also has anabolic effects in adults. It is a member of the IGF family that is comprised of three members: IGF-I, IGF-II, and insulin. IGF-I and IGF-II share approximately 50 % similarity with insulin at the amino acid level. Human IGF-I is synthesized as a 191 amino acid prepro-IGF-I, pro-IGF-I is processed to the mature, active protein, and this procedure requires glucose regulating protein 94 (GRP94) that possesses chaperone activity towards IGF-I. Deletion of muscle GRP94 impairs muscle and body growth by inhibiting local production of IGF proteins. Seven binding proteins have been described (IGFBP-1 to -7). IGFBPs extend the half life and regulate the availability of IGF-I and IGF-II. It has been described that IGFBP3 is the main circulating binding protein for IGFs, in serum approximately 75 % of circulating IGF-I and IGF-II bind to IGFBP3 and the co-carrier acid labile subunit (ALS). IGF-I binds to three receptors, and the binding to IGFIR promotes cell proliferation, survival, differentiation, and migration. IGF-I deficiency is associated to growth hormone insensitivity syndrome (GHIS) also called Laron syndrome, liver cirrhosis, age-related cardiovascular and neurological diseases, and intrauterine growth restriction.
    Molekulargewicht
    The 70 amino acid recombinant protein has a predicted molecular mass of approximately 7.6 kDa. The DTT-reduced and non-reduced protein migrate at approximately 11 kDa by SDS-PAGE. The N-terminal amino acid is Gly.
    Pathways
    RTK Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagie, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation
Sie sind hier:
Kundenservice