anti-Hund rho Guanine Nucleotide Exchange Factor (GEF) 4 Antikörper für Western Blotting

Recommended rho Guanine Nucleotide Exchange Factor (GEF) 4 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
rho Guanine Nucleotide Exchange Factor (GEF) 4 (ARHGEF4) Antikörper
  • ARHGEF4
  • ASEF
  • ASEF1
  • GEF4
  • STM6
  • 9330140K16Rik
  • Asef
  • ENSMUSG00000070955
  • Rho guanine nucleotide exchange factor (GEF) 4
  • Rho guanine nucleotide exchange factor 4
  • ARHGEF4
  • arhg4
  • Arhgef4
Epitop
N-Term
12
9
9
8
5
1
1
1
1
Reaktivität
Rind (Kuh), Hund, Meerschweinchen, Pferd, Human, Maus, Kaninchen, Ratte (Rattus)
46
1
Wirt
Kaninchen
20
16
10
Klonalität
Polyklonal
Konjugat
Unkonjugiert
7
3
3
3
3
3
Applikation
Western Blotting (WB)
42
29
9
2
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Bilder

Produktnummer ABIN2787993
358,51 €
Zzgl. Versandkosten 20,00 € und MWSt

Ähnliche anti-rho Guanine Nucleotide Exchange Factor (GEF) 4 Antikörper

Applikation / Reaktivität Human Maus Rind (Kuh) Hund Meerschweinchen Pferd Kaninchen Ratte (Rattus)
Western Blotting (WB) 43 Antikörper 2 Antikörper 1 Antikörper 1 Antikörper 1 Antikörper 1 Antikörper 1 Antikörper 1 Antikörper
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 1 Antikörper
Immunohistochemistry (IHC) 2 Antikörper
Flow Cytometry (FACS) 9 Antikörper
ELISA 29 Antikörper

General

Antigen rho Guanine Nucleotide Exchange Factor (GEF) 4 (ARHGEF4) Antikörper
Epitop N-Term
(12), (9), (9), (8), (5), (1), (1), (1), (1)
Reaktivität Rind (Kuh), Hund, Meerschweinchen, Pferd, Human, Maus, Kaninchen, Ratte (Rattus)
(46), (1)
Wirt Kaninchen
(20), (16), (10)
Klonalität
Polyklonal
Konjugat Unkonjugiert
(7), (3), (3), (3), (3), (3)
Applikation Western Blotting (WB)
(42), (29), (9), (2), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails

Antigendetails Anwendungsinformationen Handhabung Bilder
Blocking Peptide Zu diesem Produkt passt das Blocking Peptide ABIN1541270.
Sequenz ADDYEAPRAG AREADDSGPE AQSCKDQMRT NVINEILSTE RDYIKHLRDI
Homologie Cow: 79%, Dog: 93%, Guinea Pig: 92%, Horse: 86%, Human: 100%, Mouse: 86%, Rabbit: 100%, Rat: 100%
Produktmerkmale This is a rabbit polyclonal antibody against Arhgef4. It was validated on Western Blot.
Reinigung Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N-terminal region of Mouse Arhgef4

Antigendetails

Produktdetails Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung Arhgef4 (ARHGEF4 Antibody Abstract)
Hintergrund Arhgef4 acts as guanine nucleotide exchange factor (GEF) for RHOA, RAC1 and CDC42 GTPases. Binding of APC may activate RAC1 GEF activity. The APC-ARHGEF4 complex seems to be involved in cell migration as well as in E-cadherin-mediated cell-cell adhesion By similarity. It is required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Arhgef4 is involved in tumor angiogenesis and may play a role in intestinal adenoma formation and tumor progression.
Alias Symbols: 9330140K16Rik, Asef, ENSMUSG00000070955
Protein Size: 484
Molekulargewicht 57 kDa
Gen-ID 226970
NCBI Accession NM_183019, NP_898840
UniProt Q7TNR9
Forschungsgebiet Signaling, Receptors, Proteolysis / Ubiquitin, Immunology, Cytokines, Growth Factors, Inflammation
Pathways Neurotrophin Signalübertragung

Anwendungsinformationen

Produktdetails Antigendetails Handhabung Bilder zurück nach oben
Applikationshinweise Optimal working dilutions should be determined experimentally by the investigator.
Kommentare

Antigen size: 484 AA

Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails Antigendetails Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Konzentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freeze-thaw cycles.
Lagerung -20 °C
Informationen zur Lagerung For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Bilder

Produktdetails Antigendetails Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Western Blotting (WB) image for anti-rho Guanine Nucleotide Exchange Factor (GEF) 4 (ARHGEF4) (N-Term) Antikörper (ABIN2787993) Host: Rabbit
Target Name: Arhgef4
Sample Tissue: Mouse Testis lysates <...
'Independent Validation' Siegel
Antigen ARHGEF4
Chargennummer QC30159-41213
Validierte Anwendung Western Blotting
Positivkontrolle primary mouse microglia
Bewertung Passed. ABIN2787993 specifically recognizes Arhgef4 in primary mouse microglia lysates.
Primärantikörper ABIN2787993
Sekundärantikörper anti-rabbit HRP-conjugated antibodies (DakoCytomation, P0448, lot 00055814)
Protokoll
  • Primary mouse microglia are grown in DMEM (Invitrogen), supplemented with 10% foetal bovine serum (Gibco), 10% horse serum and penicillin and streptomycin (Gibco), at 37°C and 5% CO2 in a 12-well dish.
  • Lyse cells in 25µl per well cold lysis buffer (10mM Tris-HCl pH 8.0, 150mM NaCl, 1% Nonidet-P40, 1mM EDTA, 10mM NaF, 1mM Na3VO4, protease inhibitors (Sigma)).
  • Determine total protein content of the lysates using Bradford assay (Bio-Rad, 500-0006, lot 111832).
  • Denature 5µg, 10µg and 30µg total protein for 5min at 95°C in 5µl, 10µl and 20µl Laemmli SDS sample buffer and subsequently separate them on a denaturing self-made 10% SDS-PAGE for 1h at 140V.
  • Transfer proteins onto PVDF membrane (Pall Life Sciences, 75696G, lot TO3225) with a semi-dry Western blotting system for 50min at 60mA.
  • Block the membrane with TBST (TBS, 0.1% Tween) containing 5% milk for 1h at RT.
  • Wash membrane 3x 5min with TBST.
  • Incubation with primary rabbit anti-ARHGEF4 antibody (antibodies-online, ABIN2787993, lot QC30159-41213) diluted 1:500 in TBST containing 2% BSA ON at 4°C.
  • Wash membrane 5x 5min with TBST.
  • Incubation with anti-rabbit horseradish peroxidase-conjugated secondary antibodies (DakoCytomation, P0448, lot 00055814) diluted 1:2000 in TBST containing 5% milk for 1h at RT.
  • Wash membrane 5x 5min with TBST and 3x 5min with TBS.
  • Reveal protein bands using ECL Plus Western Blotting Detection Reagent (GE Healthcare, RPN2132, lot 4645641) with Curix RP2 Plus medical X-ray films (AGFA Health Care, ENKMV, lot 79040074) on an AGFA Health Care Curix60-developer.
Anmerkungen The ARHGEF4 antibody ABIN2787993 reveals multiple bands as is expected for murine Arhgef4.
Validierungsbilder
Western Blotting validation image for anti-rho Guanine Nucleotide Exchange Factor (GEF) 4 (ARHGEF4) (N-Term) antibody (ABIN2787993) Indicated amounts of total protein from primary mouse microglia cell lysate were sepa...