ARHGEF4 Antikörper (AA 143-271) (PE)

Produktnummer ABIN2749098

Produktdetails anti-ARHGEF4 Antikörper (PE)

Target: ARHGEF4 (rho Guanine Nucleotide Exchange Factor (GEF) 4 (ARHGEF4))

Bindungsspezifität: AA 143-271

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ARHGEF4 Antikörper ist konjugiert mit PE

Applikation: Flow Cytometry (FACS)

Spezifität: The mouse monoclonal antibody ARHGEF-08 recognizes human intracellular protein ARHGEF4 / ASEF1, approx. 80 kDa guanine nucleotide exchange factor specific for Rac1 and Cdc42. The epitope is located in the rough at the region of SH3 domain.

Kreuzreaktivität (Details): Human

Aufreinigung: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Recombinant fragment of human ARHGEF4 (amino acids 143-271)

Klon: ARHGEF-08

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow cytometry: Recommended dilution: 1-5 μg/mL. Intracellular staining.

Kommentare:

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate was purified by size-exclusion chromatography.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.1 mg/mL

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Details zu ARHGEF4

Target: ARHGEF4 (rho Guanine Nucleotide Exchange Factor (GEF) 4 (ARHGEF4))

Andere Bezeichnung: ARHGEF4 (ARHGEF4 Produkte)

Synonyme: ARHGEF4 antikoerper, ASEF antikoerper, ASEF1 antikoerper, GEF4 antikoerper, STM6 antikoerper, 9330140K16Rik antikoerper, Asef antikoerper, ENSMUSG00000070955 antikoerper, Rho guanine nucleotide exchange factor 4 antikoerper, uncharacterized LOC100470646 antikoerper, rho guanine nucleotide exchange factor 4 antikoerper, Rho guanine nucleotide exchange factor (GEF) 4 antikoerper, ARHGEF4 antikoerper, arhg4 antikoerper, LOC100470646 antikoerper, LOC100544917 antikoerper, Arhgef4 antikoerper

Hintergrund: Rho guanine nucleotide exchange factor 4,ARHGEF4 (Rho guanine nucleotide exchange factor 4), also known as ASEF 1 (adenomatous polyposis coli –, stimulated guanine nucleotide exchange factor 1) is an approximately 80 kDa cytoplasmic protein important for growth factor-mediated regulation of cell morphology and migration. Besides N-terminal adenomatous polyposis coli (APC)-binding region (ABR) it contains Dbl homology (DH), pleckstrin homology (PH) and SH3 domains. The SH3 domain inhibits GEF activity of ARHGEF4 by intramolecular interaction with the DH domain, whereas binding of APC stimulates the GEF activity. Activated ARHGEF4 stimulates the small GTPase Cdc42, which leads to decreased cell-cell adherence and enhanced cell migration.,ASEF1, GEF4, STM6

Gen-ID: 50649

UniProt: Q9NR80

Pathways: Neurotrophin Signalübertragung