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Nuclear extracts prepared from untreated and treated HeLa (Anisomycin) cells are diluted to 0.625 μg/well and assayed using the TransAM ATF-2 Kit. The ratio of the signals from the treated cells over the untreated must be above 4. Lot No. 25209003 was developed for 7 minutes. It gave a ratio of 4.1 (Figure 1). The endogenous level of ATF-2 expression, and this ratio may vary depending on the cell type tested and the treatment used. TransAM ATF-2 Kits are also tested for specificity in detecting ATF-2 activity. TransAM ATF-2 assays are performed in the presence of an excess of oligonucleotide containing a wild-type or mutated ATF-2 consensus binding site (Figure 2). At 20X excess, the wild-type oligonucleotide prevents ATF-2 binding to the probe immobilized on the plate. Conversely, the mutated oligonucleotide has no effect on ATF-2 binding.