Maus IL12A ELISA Kit

Recommended IL12A Product (geliefert von: Anmelden zum Anzeigen )

Antigen
Interleukin 12 alpha (IL12A) ELISA Kits
  • IL-12p35
  • Il-12a
  • Ll12a
  • p35
  • CLMF
  • IL-12A
  • NFSK
  • NKSF1
  • P35
  • IL12p35
  • Il-12 p35
  • IL12
  • interleukin 12a
  • interleukin 12A
  • interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
  • Il12a
  • IL12A
Epitop
AA 23-335, AA 23-215
Reaktivität
Maus
Alternativen
Kits mit alternativen Reaktivitäten:
13
7
7
6
5
5
3
3
2
2
2
1
1
1
Methodentyp
Sandwich ELISA
Detektionsbereich
15.6-1000 pg/mL
Untere Nachweisgrenze
15.6 pg/mL
Applikation
ELISA
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Request

Dieses Produkt umsonst

Schicken Sie uns Ihren Validierungsvorschlag. Ich möchte dieses Produkt validieren

Mehr erfahren

Bilder

Produktnummer ABIN411310
455,71 €
Zzgl. Versandkosten 20,00 € und MWSt
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Hersteller References Details
9.865132 ABIN841918 Sandwich ELISA Serum, Plasma, Tissue Homogenate 23.5-1500 pg/mL 23.5 pg/mL Anmelden zum Anzeigen
9.865132 ABIN424190 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Biological Fluids 15.62-1000 pg/mL 15.62 pg/mL Anmelden zum Anzeigen
1 ABIN5595254 Sandwich ELISA Biological Fluids, Plasma, Serum, Tissue Homogenate 0.156-10 ng/mL 0.156 ng/mL Anmelden zum Anzeigen
1 ABIN1884663 Sandwich ELISA Plasma, Serum, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Anmelden zum Anzeigen
1 ABIN455530 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 0.156-10 ng/mL 0.156 ng/mL Anmelden zum Anzeigen
1 ABIN1055172 Serum, Cell Culture Supernatant, Body Fluids, Tissue Homogenate, Plasma Anmelden zum Anzeigen
1 ABIN5596359 Sandwich ELISA Anmelden zum Anzeigen

IL12A ELISA Kit für ELISA mit den meisten Publikationen

Ähnliche IL12A ELISA Kits

Applikation / Reaktivität Huhn Rind (Kuh) Hund Ziege Meerschweinchen Pferd Human Affe Schwein Kaninchen Ratte (Rattus) Schaf Wild boar (Sus scrofa)
ELISA 2 ELISA Kits 5 ELISA Kits 6 ELISA Kits 3 ELISA Kits 1 ELISA Kits 3 ELISA Kits 13 ELISA Kits 1 ELISA Kits 5 ELISA Kits 1 ELISA Kits 7 ELISA Kits 2 ELISA Kits 2 ELISA Kits

General

Antigen Interleukin 12 alpha (IL12A) ELISA Kits
Epitop AA 23-335, AA 23-215
Reaktivität Maus
Kits mit alternativen Reaktivitäten:
(13), (7), (7), (6), (5), (5), (3), (3), (2), (2), (2), (1), (1), (1)
Methodentyp Sandwich ELISA
Detektionsbereich 15.6-1000 pg/mL
Untere Nachweisgrenze 15.6 pg/mL
Applikation ELISA
Pubmed 11 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails IL12A ELISA Kit

Antigendetails Anwendungsinformationen Handhabung ProductDetails: References for IL12A Kit (ABIN411310) Bilder
Verwendungszweck Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IL-12(p70)
Marke PicoKine™
Proben Cell Culture Supernatant, Serum
Nachweismethode Colorimetric
Analytische Methode Quantitative
Spezifität Expression system for standard: sf21
Immunogen sequence: (M23-S335)+(R23-A215)
Kreuzreaktivität (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivität <1.5pg/mL
Benötigtes Material Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: sf21
Immunogen sequence: (M23-S335)+(R23-A215)

Antigendetails

Produktdetails IL12A ELISA Kit Anwendungsinformationen Handhabung ProductDetails: References for IL12A Kit (ABIN411310) Bilder zurück nach oben
Antigen
Andere Bezeichnung IL12A (IL12A ELISA Kit Abstract)
Hintergrund

Background: Interleukin(IL)-12 is a 70-KDa cytokine comprised of two disulfide-linked proteins(p35 and p40) and is essential for the initiation of effective immune response. And the IL-12p70 is a heterodimer of p35 and p40 subunits, it is an important cytokine secreted by antigen-presenting cells in response to antigenic stimulation. Gene expression analysis of the IL-12 cytokine family subunits revealed that both strains induced high levels of p40(protein chain communal to IL-12 p70 and IL-23) as well as p19, a subunit of IL-23. Conversely only ACT- 18HS19 infection induced consistent transcription of IL-12 p35, a subunit of IL-12 p70. The standard product used in this kit is recombinant mouse IL-12 p70 comprising of a 193 amino acids p35 chain and a 313 amino acids p40 chain connected by a disulfide bond.

Synonyms: Interleukin-12 subunit alpha ,Il12a ,

Full Gene Name: interleukin 12A

Gen-ID 16159
UniProt F8WI71
Pathways JAK-STAT Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation

Anwendungsinformationen

Produktdetails IL12A ELISA Kit Antigendetails Handhabung ProductDetails: References for IL12A Kit (ABIN411310) Bilder zurück nach oben
Applikationshinweise Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plattentyp Pre-coated
Protokoll mouse IL-12(P70) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-12(P70) has been precoated onto 96-well plates. Standards(sf21, (M23-S335)+(R23-A215)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-12(P70) is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-12(P70) amount of sample captured in plate.
Testdurchführung Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse IL-12(P70) standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse IL-12(P70) standard solution and each sample be measured in duplicate.
Testpräzision
  • Sample 1: n=16, Mean(pg/ml): 97, Standard deviation: 6.5, CV(%): 6.7
  • Sample 2: n=16, Mean(pg/ml): 367, Standard deviation: 25.7, CV(%): 7
  • Sample 3: n=16, Mean(pg/ml): 633, Standard deviation: 34.2, CV(%): 5.4,
  • Sample 1: n=24, Mean(pg/ml): 123, Standard deviation: 9.23, CV(%): 7.5
  • Sample 2: n=24, Mean(pg/ml): 408, Standard deviation: 33.05, CV(%): 8.1
  • Sample 3: n=24, Mean(pg/ml): 657, Standard deviation: 38.8, CV(%): 5.9
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails IL12A ELISA Kit Antigendetails Anwendungsinformationen ProductDetails: References for IL12A Kit (ABIN411310) Bilder zurück nach oben
Handhabung Avoid multiple freeze-thaw cycles.
Lagerung -20 °C,4 °C
Informationen zur Lagerung Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Haltbarkeit 12 months

ProductDetails: References for IL12A Kit (ABIN411310)

Produktdetails IL12A ELISA Kit Antigendetails Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Li, Li, Liu, Zhuang, Yang, Zhang: "Intranasal immunization with recombinant Lactococci carrying human papillomavirus E7 protein and mouse interleukin-12 DNA induces E7-specific antitumor effects in C57BL/6 mice." in: Oncology letters, Vol. 7, Issue 2, pp. 576-582, 2014

Yu, Liu, Chen, Zeng: "Anti-inflammatory effect of Qingwen Baidu Decoction (?????) in sepsis rats." in: Chinese journal of integrative medicine, Vol. 20, Issue 12, pp. 934-43, 2014

Pan, Wang: "Synergistic interaction between choline and aspirin against acute inflammation induced by carrageenan and lipopolysaccharide." in: International immunopharmacology, Vol. 20, Issue 1, pp. 229-37, 2014

Li, Li, Zuo, Zhou, Hao, Dang, Jiang, He, Deng: "Up-regulated expression of Tim-3/Gal-9 at maternal-fetal interface in pregnant woman with recurrent spontaneous abortion." in: Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban, Vol. 34, Issue 4, pp. 586-90, 2014

Ma, He, Han, Yang, Ji: "A clinical study of HBsAg-activated dendritic cells and cytokine-induced killer cells during the treatment for chronic hepatitis B." in: Scandinavian journal of immunology, Vol. 78, Issue 4, pp. 387-93, 2013

Huang, Yang, Jiang, Shao, Sun, Chen, Dong, Zhang: "Anti-tumor immune responses of tumor-associated macrophages via toll-like receptor 4 triggered by cationic polymers." in: Biomaterials, Vol. 34, Issue 3, pp. 746-55, 2012

Huang, Zhang, Jiang, Zhang, Chen, Dong, Zhang: "Targeted delivery of oligonucleotides into tumor-associated macrophages for cancer immunotherapy." in: Journal of controlled release : official journal of the Controlled Release Society, Vol. 158, Issue 2, pp. 286-92, 2012

Gui, Xiong, Yang, Li, Huang: "Effects of acupuncture on LIF and IL-12 in rats of implantation failure." in: American journal of reproductive immunology (New York, N.Y. : 1989), Vol. 67, Issue 5, pp. 383-90, 2012

Li, Li, Li, Deng, Tian, Jiang, Wang, Wang: "A rat model for stable chronic obstructive pulmonary disease induced by cigarette smoke inhalation and repetitive bacterial infection." in: Biological & pharmaceutical bulletin, Vol. 35, Issue 10, pp. 1752-60, 2012

Hara, Mimura, Abe, Shioi, Seiki, Sakamoto: "Deletion of the Mint3/Apba3 gene in mice abrogates macrophage functions and increases resistance to lipopolysaccharide-induced septic shock." in: The Journal of biological chemistry, Vol. 286, Issue 37, pp. 32542-51, 2011

Allgemeine Veröffentlichungen

Shi, Chen, Wang, Zhu, Xu, Wang, Xiao, Shi, Zhang, Xu: "Short amyloid-beta immunogens show strong immunogenicity and avoid stimulating pro-inflammatory pathways in bone marrow-derived dendritic cells from C57BL/6J mice in vitro." in: Peptides, Vol. 32, Issue 8, pp. 1617-25, 2011

Bilder

Produktdetails IL12A ELISA Kit Antigendetails Anwendungsinformationen Handhabung ProductDetails: References for IL12A Kit (ABIN411310) zurück nach oben
Bilder des Herstellers
ELISA image for IL12A ELISA Kit (Interleukin 12 alpha) (ABIN411310) Mouse IL-12(p70) PicoKine ELISA Kit standard curve