IL12A ELISA Kit (Interleukin 12 alpha)

Details for Product IL12A ELISA Kit No. ABIN5692238, Anbieter: Anmelden zum Anzeigen
Antigen
  • IL-12p35
  • Il-12a
  • Ll12a
  • p35
  • CLMF
  • IL-12A
  • NFSK
  • NKSF1
  • P35
  • IL12p35
  • Il-12 p35
  • IL12
  • interleukin 12a
  • interleukin 12A
  • Il12a
  • IL12A
Epitop
AA 23-215, AA 23-335
Reaktivität
Ratte (Rattus)
Alternativen
Kits mit alternativen Reaktivitäten:
15
11
10
7
6
6
4
4
3
2
2
2
1
1
1
Methodentyp
Sandwich ELISA
Detektionsbereich
15.6 pg/mL - 1000 pg/mL
Untere Nachweisgrenze
15.6 pg/mL
Applikation
ELISA
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Verwendungszweck Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat IL-12(P70). 96wells/kit, with removable strips.
Marke PicoKine™
Proben Cell Culture Supernatant, Serum
Analytische Methode Quantitative
Nachweismethode Colorimetric
Spezifität Expression system for standard: sf21, Immunogen sequence: (M23-S335)+(R23-S215)
Sensitivität < 1.5 pg/mL
Bestandteile 96-well plate precoated with antibody
lyophilized recombinant standard
biotinylated antibody (dilution 1:100)
Avidin-Biotin-Peroxidase Complex(ABC)(dilution 1:100)
Sample diluent buffer
Antibody diluent buffer
ABC diluent buffer
TMB color developing agent
TMB stop solution
Adhesive cover
Plasmids, Primers & others Plasmide, Primers & weitere IL12A products on genomics-online (e.g. as negative or positive controls)
Andere Bezeichnung Il12a (IL12A ELISA Kit Abstract)
Hintergrund

Synonyms: Interleukin-12 subunit alpha, IL-12A, Cytotoxic lymphocyte maturation factor 35 kDa subunit, CLMF p35, IL-12 subunit p35, Il12a

Background: Interleukin (IL)-12 is a 70-KDa cytokine comprised of two disulfide-linked proteins (p35 and p40) and is essential for the initiation of effective immune response. And the IL-12p70 is a heterodimer of p35 and p40 subunits, it is an important cytokine secreted by antigen-presenting cells in response to antigenic stimulation. Gene expression analysis of the IL-12 cytokine family subunits revealed that both strains induced high levels of p40 (protein chain communal to IL-12 p70 and IL-23) as well as p19, a subunit of IL-23. Conversely only ACT- 18HS19 infection induced consistent transcription of IL-12 p35, a subunit of IL-12 p70. The standard product used in this kit is recombinant rat IL-12 p70 comprising of a 193 amino acids p35 chain and a 313 amino acids p40 chain connected by a disulfide bond.

Cellular Localisation: Secreted.
UniProt Q9R103
Pathways JAK-STAT Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
Testdauer 15 min
Plattentyp Pre-coated
Beschränkungen Nur für Forschungszwecke einsetzbar
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Haltbarkeit 12 months
Bilder des Herstellers
ELISA image for Interleukin 12 alpha (IL12A) ELISA Kit (ABIN5692238) Rat IL-12P70 PicoKine ELISA Kit standard curve
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