anti-Maus PARP1 Antikörper für Enzyme Immunoassay

Recommended PARP1 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Poly (ADP-Ribose) Polymerase 1 (PARP1) Antikörper
  • BEST:LD21673
  • CG17685
  • CG17696
  • CG17718
  • CG40411
  • D.PARP
  • Dm.pARTa
  • Dmel\\CG40411
  • LD21673.3prime
  • PARP
  • PARP-1
  • PARP1
  • dPARP
  • parp
  • ADPRT
  • ADPRT 1
  • ADPRT1
  • ARTD1
  • PPOL
  • pADPRT-1
  • Adprt
  • Parp-1
  • adprt1
  • padprt-1
  • ppol
  • 5830444G22Rik
  • AI893648
  • Adprp
  • Adprt1
  • C80510
  • parp-1
  • sPARP-1
  • Poly-(ADP-ribose) polymerase
  • poly (ADP-ribose) polymerase 1
  • poly (ADP-ribose) polymerase family, member 1
  • poly ADP-ribose polymerase
  • Parp
  • PARP1
  • Parp1
  • parp1
Reaktivität
Fruchtfliege (Drosophila melanogaster), Hamster, Human, Maus, Ratte (Rattus), Xenopus laevis
387
83
79
18
17
13
9
9
6
5
4
3
2
2
1
1
1
1
1
Wirt
Maus
378
47
1
Klonalität
Monoklonal
Konjugat
Dieser PARP1 Antikörper ist unkonjugiert
25
24
24
24
24
23
Applikation
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
237
233
164
50
45
40
31
23
13
12
5
4
4
3
2
1
1
1
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Produktnummer ABIN118984
$ 500.50
Zzgl. Versandkosten $45.00

General

Antigen Poly (ADP-Ribose) Polymerase 1 (PARP1) Antikörper
Reaktivität Fruchtfliege (Drosophila melanogaster), Hamster, Human, Maus, Ratte (Rattus), Xenopus laevis
(387), (83), (79), (18), (17), (13), (9), (9), (6), (5), (4), (3), (2), (2), (1), (1), (1), (1), (1)
Wirt Maus
(378), (47), (1)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser PARP1 Antikörper ist unkonjugiert
(25), (24), (24), (24), (24), (23)
Applikation Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(237), (233), (164), (50), (45), (40), (31), (23), (13), (12), (5), (4), (4), (3), (2), (1), (1), (1)
Pubmed 4 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-PARP1 Antikörper

Target Details PARP1 Anwendungsinformationen Handhabung Referenzen für anti-PARP1 Antikörper (ABIN118984) Bilder
Spezifität This antibody recognises poly (ADP-ribose) polymerase (PARP).
Kreuzreaktivität (Details) Species reactivity (expected):Xenopus, Drosophila, Hamster, Rat and Mouse.
Species reactivity (tested):Human.
Reinigung Affinity Chromatography on Protein G.
Immunogen Human PARP. Spleen cells from immunised BALB/c mice were fused with cells of mouse NSO myeloma cell line.
Klon A6-4-12
Isotyp IgG1

Target Details PARP1

Produktdetails anti-PARP1 Antikörper Anwendungsinformationen Handhabung Referenzen für anti-PARP1 Antikörper (ABIN118984) Bilder zurück nach oben
Antigen
Andere Bezeichnung PARP1 (PARP1 Antibody Abstract)
Hintergrund PARP (Poly (ADP-ribose) polymerase) is a 113 kDa nuclear protein which can exist as a homo- or hetero-dimer, and is strongly activated by DNA strand breaks. This protein acts as a molecular ''nick sensor'' and functions in base excision repair, poly(ADPribosyl)ation of acceptor proteins involved in chromatin architecture and DNA metabolism, and participates in protein modification to enhance or repress transcription. PARP also plays a role in other cellular processes, including cell proliferation and differentiation. PARP is ribosylated by PARP2, and during apoptosis, ICE family members, such as caspase 3 and 7, cleave PARP to yield an 85 kDa and a 25 kDa fragment. PARP cleavage is considered to be one of the classical characteristics of apoptosis. PARP interacts with proteins in the base excision repair complex containing at least XRCC1, PARP2, POLB and LIG3. In addition PARP forms heterodimers with PARP2, and interacts with PARP3.Synonyms: ADPRT, ADPRT 1, NAD(+) ADP-ribosyltransferase 1, PARP-1, PPOL, Poly [ADP-ribose] polymerase 1, Poly[ADP-ribose] synthetase 1
Gen-ID 142
NCBI Accession NP_001609
UniProt P09874
Pathways Apoptose, Caspase Kaskade in der Apoptose, DNA Reparatur, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location

Anwendungsinformationen

Produktdetails anti-PARP1 Antikörper Target Details PARP1 Handhabung Referenzen für anti-PARP1 Antikörper (ABIN118984) Bilder zurück nach oben
Applikationshinweise Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections, Sodium citrate buffer pH 6.0 is recommended for this purpose. ELISA. Immunoprecipitation. Western Blot: 1/1000 - 1/5000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-PARP1 Antikörper Target Details PARP1 Anwendungsinformationen Referenzen für anti-PARP1 Antikörper (ABIN118984) Bilder zurück nach oben
Konzentration 1.0 mg/mL
Handhabung Avoid repeated freezing and thawing.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.

Referenzen für anti-PARP1 Antikörper (ABIN118984)

Produktdetails anti-PARP1 Antikörper Target Details PARP1 Anwendungsinformationen Handhabung Bilder zurück nach oben
Allgemeine Veröffentlichungen

Bie, Yuan, Zhao, Zhang: "Method for estimating the axial intensity derivative in the TIE with higher order intensity derivatives and noise suppression." in: Optics express, Vol. 20, Issue 7, pp. 8186-91, 2012

Harris, Jakob, Taucher-Scholz, Dianov, Becherel, Lavin: "Aprataxin, poly-ADP ribose polymerase 1 (PARP-1) and apurinic endonuclease 1 (APE1) function together to protect the genome against oxidative damage." in: Human molecular genetics, Vol. 18, Issue 21, pp. 4102-17, 2009

Freire, dAdda Di Fagagna, Wu, Pedrazzi, Stagljar, Hickson, Jackson: "Cleavage of the Bloom's syndrome gene product during apoptosis by caspase-3 results in an impaired interaction with topoisomerase IIIalpha." in: Nucleic acids research, Vol. 29, Issue 15, pp. 3172-80, 2001

Krohn, Preis, Prehn: "Staurosporine-induced apoptosis of cultured rat hippocampal neurons involves caspase-1-like proteases as upstream initiators and increased production of superoxide as a main downstream effector." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 18, Issue 20, pp. 8186-97, 1998