PARP1 Antikörper (C-Term)

Produktnummer ABIN5596914

Produktdetails anti-PARP1 Antikörper

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Bindungsspezifität: C-Term

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PARP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Kreuzreaktivität (Details): This antibody is specific for human PARP1 protein. No cross reactivity detected towards other PARP members when using siRNAs against 18 PARP family members. Cross-reactivity with PARP1 from other sources has not been determined.

Aufreinigung: PARP1 (internal) was purified from monospecific antiserum by immunoaffinity chromatography using protein A coupled to agarose beads.

Immunogen:

Immunogen: PARP1 (internal) purified antibody was prepared from whole rabbit serum produced by repeated immunizations with c-terminus region of human PARP1 autocatalytic domain recombinant protein.

Immunogen Type: Recombinant Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-PARP1 (internal) antibody has been validated by western blotting and nanoimmunoassay (NIA). Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 113 kDa in size corresponding to PARP-1 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu PARP1

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Andere Bezeichnung: PARP1 (PARP1 Produkte)

Synonyme: ADPRT antikoerper, ADPRT 1 antikoerper, ADPRT1 antikoerper, ARTD1 antikoerper, PARP antikoerper, PARP-1 antikoerper, PPOL antikoerper, pADPRT-1 antikoerper, Adprt antikoerper, Parp-1 antikoerper, 5830444G22Rik antikoerper, AI893648 antikoerper, Adprp antikoerper, Adprt1 antikoerper, C80510 antikoerper, parp-1 antikoerper, sPARP-1 antikoerper, BEST:LD21673 antikoerper, CG17685 antikoerper, CG17696 antikoerper, CG17718 antikoerper, CG40411 antikoerper, D.PARP antikoerper, Dm.pARTa antikoerper, Dmel\\CG40411 antikoerper, LD21673.3prime antikoerper, PARP1 antikoerper, dPARP antikoerper, parp antikoerper, adprt1 antikoerper, padprt-1 antikoerper, ppol antikoerper, si:dkey-206f10.3 antikoerper, wu:fc60f12 antikoerper, zgc:110092 antikoerper, poly(ADP-ribose) polymerase 1 antikoerper, poly (ADP-ribose) polymerase 1 antikoerper, poly (ADP-ribose) polymerase family, member 1 antikoerper, Poly-(ADP-ribose) polymerase antikoerper, poly(ADP-ribose) polymerase 1 L homeolog antikoerper, PARP1 antikoerper, Parp1 antikoerper, Parp antikoerper, parp1.L antikoerper, parp1 antikoerper

Hintergrund:

Synonyms: Poly [ADP-ribose] polymerase 1, ADP-ribosyltransferase diphtheria toxin-like 1, ARTD1, NAD(+) ADP-ribosyltransferase 1, ADPRT 1, PPOL

Background: PARP1 is the primary member of the poly(ADP-ribose) polymerase family, whose function is to signal DNA damage (and to recruit repair proteins) by PARylation. PARP1 is also involved in multiple cell death pathways, including apoptosis, necroptosis, autophagy, and a relatively new pathway termed parthanatos. It has been implicated in a new form of cell death termed parthanatos. PARP1 can also promote tissue survival by shifting the balance of cell death programs between autophagy and necrosis. Clinical studies have shown vulnerability to PARP inhibitors in DNA repair defective cancers. Anti-PARP1 (internal) antibody is useful for researchers interested in cellular processes including DNA damage, transcriptional control, and stem cell identity research.

Gene Name: PARP1

UniProt: P09874

Pathways: Apoptose, Caspase Kaskade in der Apoptose, DNA Reparatur, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location