anti-Maus Caspase 3 Antikörper für Immunofluorescence

Recommended Caspase 3 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) Antikörper
  • CC3
  • CG14902
  • Cas3
  • Casp 3
  • Casp3
  • Caspase-3
  • DECAY
  • Decay
  • Dmel\\CG14902
  • Drosophila executioner caspase related to Apopain/Yama
  • caspase 3
  • caspase-3
  • CG7788
  • DRICE
  • Dmel\\CG7788
  • DrICE
  • DrIce
  • Drice
  • ICE
  • crice
  • drICE
  • drIce
  • drice
  • ice
  • A830040C14Rik
  • AC-3
  • Apopain
  • CPP32
  • Lice
  • Yama
  • mldy
  • CPP32B
  • SCA-1
  • xcpp32
  • caspase 3, apoptosis-related cysteine peptidase
  • death executioner caspase related to Apopain/Yama
  • CG7788 gene product from transcript CG7788-RA
  • caspase 3
  • CASP3
  • decay
  • Ice
  • Casp3
  • casp3
Epitop
AA 25-145
41
32
27
24
22
21
19
19
16
15
12
12
11
10
10
10
9
7
5
5
5
5
5
4
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Maus
567
304
254
48
47
38
29
28
24
20
18
18
18
12
9
8
7
4
2
2
Wirt
Maus
458
161
19
4
2
Klonalität
Monoklonal
Konjugat
Dieser Caspase 3 Antikörper ist unkonjugiert
27
24
19
14
9
9
9
6
4
4
4
4
4
4
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
Applikation
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
482
238
219
147
95
77
55
50
44
29
28
13
9
9
7
5
4
3
2
2
2
2
2
2
2
1
1
1
1
Optionen
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Produktnummer ABIN968341
146,73 €
Zzgl. Versandkosten 20,00 € und MWSt
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
12.326117 ABIN2451932 IC IF ELISA WB Rabbit Cleavage Site, Subunit p17/p20, activated Anmelden zum Anzeigen Polyclonal 4
12.326117 ABIN4288099 ICC IF IHC IHC (p) WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 1
12.326117 ABIN2705682 IC IF IHC WB Rabbit Center Anmelden zum Anzeigen Polyclonal
12.326117 ABIN4288100 ICC IF IHC IHC (fro) IHC (p) IP WB Rabbit IgG Center Anmelden zum Anzeigen Polyclonal
12.326117 ABIN1871461 IF IHC WB Rabbit IgG Anmelden zum Anzeigen Polyclonal
12.326117 ABIN1871462 IF IHC IP WB Rabbit IgG Anmelden zum Anzeigen Polyclonal
12.326117 ABIN2405186 IF IP WB Rabbit IgG Anmelden zum Anzeigen Polyclonal
1 ABIN269423 ICC IF IHC (p) IHC Rabbit IgG Anmelden zum Anzeigen Polyclonal 10
1 ABIN252651 CyTOF EM FACS ICC IF IHC IHC (fro) IHC (p) SimWes WB Mouse IgG1 kappa Anmelden zum Anzeigen 31A1067 107
1 ABIN272003 IF IHC (p) WB Rabbit Anmelden zum Anzeigen Polyclonal
1 ABIN968342 IF IP WB Mouse IgG1 AA 25-145 Anmelden zum Anzeigen 46-Caspase 5
1 ABIN2787903 IF WB Rabbit C-Term Anmelden zum Anzeigen Polyclonal
1 ABIN3183667 ELISA IF IHC WB Rabbit IgG Ser1981 Anmelden zum Anzeigen Polyclonal
1 ABIN4288106 FACS ICC IF IHC (fro) IHC (p) WB DyLight 488 Mouse IgG1 kappa Anmelden zum Anzeigen 31A1067 1
1 ABIN4288120 ICC IF IHC IHC (fro) IHC (p) WB Mouse IgG1 kappa Anmelden zum Anzeigen 31A1067
1 ABIN2891760 IF IHC (p) WB Rabbit Asp146 Anmelden zum Anzeigen Polyclonal
1 ABIN4288090 CyTOF EM FACS ICC IF IHC IHC (fro) IHC (p) SimWes WB Mouse IgG1 kappa Anmelden zum Anzeigen 31A1067
1 ABIN4288108 FACS ICC IF IHC (fro) IHC (p) WB DyLight 755 Mouse IgG1 kappa Anmelden zum Anzeigen 31A1067
1 ABIN4288115 FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 405 Mouse IgG1 kappa Anmelden zum Anzeigen 31A1067
1 ABIN4288091 ICC IF IHC IHC (p) Rabbit IgG Anmelden zum Anzeigen Polyclonal

anti-Caspase 3 Antikörper für Immunofluorescence mit den meisten Publikationen

Ähnliche anti-Caspase 3 Antikörper

Applikation / Reaktivität Maus
BioImaging (BI) 1 Antikörper
Chromatin Immunoprecipitation (ChIP) 2 Antikörper
Cytometry by Time of Flight (CyTOF) 3 Antikörper
Dot Blot (DB) 35 Antikörper
Electron Microscopy (EM) 2 Antikörper
ELISA 92 Antikörper
Enzyme Immunoassay (EIA) 3 Antikörper
Flow Cytometry (FACS) 40 Antikörper
Immunochromatography (IC) 3 Antikörper
Immunocytochemistry (ICC) 30 Antikörper
Immunofluorescence (IF) 38 Antikörper
Immunofluorescence (Cultured Cells) (IF (cc)) 13 Antikörper
Immunofluorescence (fixed cells) (IF/ICC) 4 Antikörper
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 29 Antikörper
Immunohistochemistry (IHC) 92 Antikörper
Immunohistochemistry (Frozen Sections) (IHC (fro)) 25 Antikörper
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 74 Antikörper
Immunoprecipitation (IP) 35 Antikörper
Intracellular Staining (ICS) 2 Antikörper

General

Antigen Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) Antikörper
Epitop AA 25-145
(41), (32), (27), (24), (22), (21), (19), (19), (16), (15), (12), (12), (11), (10), (10), (10), (9), (7), (5), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Maus
(567), (304), (254), (48), (47), (38), (29), (28), (24), (20), (18), (18), (18), (12), (9), (8), (7), (4), (2), (2)
Wirt Maus
(458), (161), (19), (4), (2)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser Caspase 3 Antikörper ist unkonjugiert
(27), (24), (19), (14), (9), (9), (9), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Applikation Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(482), (238), (219), (147), (95), (77), (55), (50), (44), (29), (28), (13), (9), (9), (7), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Pubmed 5 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-Caspase 3 Antikörper

Target Details Caspase 3 Anwendungsinformationen Handhabung ProductDetails: References for anti-Caspase 3 antibody (ABIN968341) Bilder
Produktmerkmale 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Reinigung The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Mouse CPP32 aa. 25-145
Klon 46-Caspase
Isotyp IgG1

Target Details Caspase 3

Produktdetails anti-Caspase 3 Antikörper Anwendungsinformationen Handhabung ProductDetails: References for anti-Caspase 3 antibody (ABIN968341) Bilder zurück nach oben
Antigen
Andere Bezeichnung Caspase-3 (CASP3 Antibody Abstract)
Hintergrund Apoptosis, a selective process of genetically programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptotic cells are characterized by loss of cell volume, plasma membrane blebbing, nuclear condensation, chromatin aggregation, and endonucleocytic degradation of DNA into nucleosomal fragments. Caspase-3 (CPP32, Yama, apopain) is a member of the family of cysteine proteases which includes interleukin-1beta-converting enzyme (ICE) and C. elegans protein, Ced-3. An apoptotic signal such as granzyme B of cytotoxic T-cells (CTLs) or ICE-like proteases induces the intracellular cleavage of Caspase-3 from the inactive proform (32kDa) to the active form which consists of the p20, p17, and p12 subunits. The active form of Caspase-3 cleaves several other apoptotic proteins including proteins such as DNA fragmentation factor (DFF). Apoptosis can be inhibited by coexpression of Bcl-2 as well as inhibitors of Caspase-3 or other members of the family of cysteine proteases. This antibody recognizes the mouse 32 kDa pro-Caspase-3 and the p17 cleaved form of Caspase 3 in T cell lymphocytes treated with camptothecin.
Synonyms: CPP32
Molekulargewicht 32 kDa
Forschungsgebiet Apoptosis/Necrosis, Autophagy
Pathways Apoptose, Caspase Kaskade in der Apoptose, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation

Anwendungsinformationen

Produktdetails anti-Caspase 3 Antikörper Target Details Caspase 3 Handhabung ProductDetails: References for anti-Caspase 3 antibody (ABIN968341) Bilder zurück nach oben
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-Caspase 3 Antikörper Target Details Caspase 3 Anwendungsinformationen ProductDetails: References for anti-Caspase 3 antibody (ABIN968341) Bilder zurück nach oben
Format Liquid
Konzentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store undiluted at -20°C.

ProductDetails: References for anti-Caspase 3 antibody (ABIN968341)

Produktdetails anti-Caspase 3 Antikörper Target Details Caspase 3 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Li, Zhao, He, Kim, Kuharsky, Rabinowich, Chen, Du, Yin: "Relief of extrinsic pathway inhibition by the Bid-dependent mitochondrial release of Smac in Fas-mediated hepatocyte apoptosis." in: The Journal of biological chemistry, Vol. 277, Issue 30, pp. 26912-20, 2002

Jia, Patwari, Srinivasula, Newland, Fernandes-Alnemri, Alnemri, Kelsey: "Bax translocation is crucial for the sensitivity of leukaemic cells to etoposide-induced apoptosis." in: Oncogene, Vol. 20, Issue 35, pp. 4817-26, 2001

Hatai, Matsuzawa, Inoshita, Mochida, Kuroda, Sakamaki, Kuida, Yonehara, Ichijo, Takeda: "Execution of apoptosis signal-regulating kinase 1 (ASK1)-induced apoptosis by the mitochondria-dependent caspase activation." in: The Journal of biological chemistry, Vol. 275, Issue 34, pp. 26576-81, 2000

Allgemeine Veröffentlichungen

Liu, Kim, Yang, Jemmerson, Wang: "Induction of apoptotic program in cell-free extracts: requirement for dATP and cytochrome c." in: Cell, Vol. 86, Issue 1, pp. 147-57, 1996

Fernandes-Alnemri, Litwack, Alnemri: "CPP32, a novel human apoptotic protein with homology to Caenorhabditis elegans cell death protein Ced-3 and mammalian interleukin-1 beta-converting enzyme." in: The Journal of biological chemistry, Vol. 269, Issue 49, pp. 30761-4, 1995

Bilder

Produktdetails anti-Caspase 3 Antikörper Target Details Caspase 3 Anwendungsinformationen Handhabung ProductDetails: References for anti-Caspase 3 antibody (ABIN968341) zurück nach oben
Bilder des Herstellers
Western Blotting (WB) image for anti-Caspase 3 Antikörper (Caspase 3, Apoptosis-Related Cysteine Peptidase) (AA 25-145) (ABIN968341) Western blot analysis of Caspase-3 on RSV-3T3 lysate. Lane 1: 1:1000, lane 2: 1:2000,...
Immunofluorescence (IF) image for anti-Caspase 3 Antikörper (Caspase 3, Apoptosis-Related Cysteine Peptidase) (AA 25-145) (ABIN968341) Immunofluorescence staining of RSV-3T3 cells.