alpha Adaptin Antikörper (AA 38-215)
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- Target Alle alpha Adaptin (AP2A1) Antikörper anzeigen
- alpha Adaptin (AP2A1) (Adaptor-Related Protein Complex 2, alpha 1 Subunit (AP2A1))
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Bindungsspezifität
- AA 38-215
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Reaktivität
- Human, Maus, Ratte, Hund
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser alpha Adaptin Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), BioImaging (BI)
- Kreuzreaktivität
- Ratte (Rattus), Human, Hund
- Produktmerkmale
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States. - Aufreinigung
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Immunogen
- Mouse Adaptin alpha [A] aa. 38-215
- Klon
- 8-Adaptin alpha
- Isotyp
- IgG1
- Top Product
- Discover our top product AP2A1 Primärantikörper
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- Kommentare
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Related Products: ABIN968545, ABIN967389
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 250 μg/mL
- Buffer
- Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Store undiluted at -20°C.
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Endocytosis of synaptotagmin 1 is mediated by a novel, tryptophan-containing motif." in: Traffic (Copenhagen, Denmark), Vol. 4, Issue 7, pp. 468-78, (2003) (PubMed).
: "Enthoprotin: a novel clathrin-associated protein identified through subcellular proteomics." in: The Journal of cell biology, Vol. 158, Issue 5, pp. 855-62, (2002) (PubMed).
: "Numb is an endocytic protein." in: The Journal of cell biology, Vol. 151, Issue 6, pp. 1345-52, (2001) (PubMed).
: "The tyrosine kinase ACK1 associates with clathrin-coated vesicles through a binding motif shared by arrestin and other adaptors." in: The Journal of biological chemistry, Vol. 276, Issue 21, pp. 18392-8, (2001) (PubMed).
: "Cloning of cDNAs encoding two related 100-kD coated vesicle proteins (alpha-adaptins)." in: The Journal of cell biology, Vol. 108, Issue 3, pp. 833-42, (1989) (PubMed).
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Endocytosis of synaptotagmin 1 is mediated by a novel, tryptophan-containing motif." in: Traffic (Copenhagen, Denmark), Vol. 4, Issue 7, pp. 468-78, (2003) (PubMed).
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- Target
- alpha Adaptin (AP2A1) (Adaptor-Related Protein Complex 2, alpha 1 Subunit (AP2A1))
- Andere Bezeichnung
- Adaptin alpha (AP2A1 Produkte)
- Synonyme
- ADTAA antikoerper, AP2-ALPHA antikoerper, CLAPA1 antikoerper, adtaa antikoerper, clapa1 antikoerper, ap2-alpha antikoerper, AP2A1 antikoerper, Adtaa antikoerper, adaptor related protein complex 2 alpha 1 subunit antikoerper, adaptor-related protein complex 2, alpha 1 subunit antikoerper, AP-2 complex subunit alpha-1 antikoerper, AP2A1 antikoerper, ap2a1 antikoerper, Ap2a1 antikoerper, EDI_093420 antikoerper, EDI_259990 antikoerper
- Hintergrund
- Sorting of integral membrane proteins at various stages of the endocytic and secretory pathways is mediated by vesicular trafficking between a variety of organelles. Two sorting signals are tyrosine-based and dileucine-based signals that interact with heterotetrameric adaptor protein complexes (AP-1, AP-2, AP-3, and AP-4), which are associated with the vesicle coats. These coatomers contain two large Adaptin proteins (alpha, gamma, delta, or epsilon and beta1, beta2, beta3, or beta4, respectively) that are noncovalently linked to one medium chain (µ1, µ2, µ3, or µ4) and one small chain (sigma1, sigma2, sigma3, or sigma4). The AP-1 and AP-3 complexes are involved in protein sorting from the TGN and endosomes, while AP-2 adaptor complexes are involved in clathrin-mediated endocytosis. In the AP-2 complex, Adaptin alpha is expressed in two very similar isoforms from two different genes. Adaptin alpha [A] (112kDa) is expressed primarily in brain, while Adaptin alpha [C] (105kDa) is expressed in brain, liver and other tissues. This antibody is routinely tested by western blot analysis.
- Molekulargewicht
- 112 kDa
- Pathways
- Notch Signalweg, EGFR Signaling Pathway, Neurotrophin Signalübertragung, EGFR Downregulation
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