MLH1 Antikörper
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- Target Alle MLH1 Antikörper anzeigen
- MLH1 (MutL Homolog 1 (MLH1))
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Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser MLH1 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Marke
- BD Pharmingen™
- Produktmerkmale
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Please refer to us for technical protocols. - Aufreinigung
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Immunogen
- Human recombinant MLH
- Klon
- G168
- Isotyp
- IgG1
- Top Product
- Discover our top product MLH1 Primärantikörper
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- Applikationshinweise
- Applications include western blot analysis (0.5-2.0 µg/ml) and immunohistochemical staining of frozen and paraffin-embedded tissue sections (5-20 µg/ml). Jurkat control lysate [50 µg (1 µg/µl)] is provided as a western blot control (store lysate at -20°C). Additional Jurkat control lysate (ABIN968537) is sold separately. Intestine or normal colon is suggested as a positive control for immunohistochemical staining. In intestine, staining is primarily nuclear and is seen in the crypts of Lieberkuhn, similar to that described in the literature. Both nuclear and cytoplasmic staining have been observed in a variety of other normal and tumor tissue and cell types. Clone G168-728 (ABIN967392) is recommended for immunoprecipitation of MLH1.
- Kommentare
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Related Products: ABIN968537, ABIN967392, ABIN967389
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 0.25 mg/mL
- Buffer
- Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Store undiluted at -20°C.
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Involvement of mouse Mlh1 in DNA mismatch repair and meiotic crossing over." in: Nature genetics, Vol. 13, Issue 3, pp. 336-42, (1996) (PubMed).
: "Restoration of mismatch repair to nuclear extracts of H6 colorectal tumor cells by a heterodimer of human MutL homologs." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 6, pp. 1950-4, (1995) (PubMed).
: "It was a very good year for DNA repair." in: Cell, Vol. 76, Issue 1, pp. 1-4, (1994) (PubMed).
: "Binding of mismatched microsatellite DNA sequences by the human MSH2 protein." in: Science (New York, N.Y.), Vol. 266, Issue 5189, pp. 1403-5, (1994) (PubMed).
: "Dual requirement in yeast DNA mismatch repair for MLH1 and PMS1, two homologs of the bacterial mutL gene." in: Molecular and cellular biology, Vol. 14, Issue 1, pp. 407-15, (1994) (PubMed).
: "MLH1, PMS1, and MSH2 interactions during the initiation of DNA mismatch repair in yeast." in: Science (New York, N.Y.), Vol. 265, Issue 5175, pp. 1091-3, (1994) (PubMed).
: "Escherichia coli mutS-encoded protein binds to mismatched DNA base pairs." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 14, pp. 5057-61, (1986) (PubMed).
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Involvement of mouse Mlh1 in DNA mismatch repair and meiotic crossing over." in: Nature genetics, Vol. 13, Issue 3, pp. 336-42, (1996) (PubMed).
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- Target
- MLH1 (MutL Homolog 1 (MLH1))
- Andere Bezeichnung
- MLH1 (MLH1 Produkte)
- Synonyme
- CG11482 antikoerper, Dmel\\CG11482 antikoerper, dmlh-1 antikoerper, dmlh1 antikoerper, zgc:66301 antikoerper, MLH1 antikoerper, LOC100232198 antikoerper, 1110035C23Rik antikoerper, AI317206 antikoerper, AI325952 antikoerper, AI561766 antikoerper, COCA2 antikoerper, FCC2 antikoerper, HNPCC antikoerper, HNPCC2 antikoerper, hMLH1 antikoerper, mutL homolog 1 antikoerper, CG11482 gene product from transcript CG11482-RB antikoerper, mutL homolog 1 S homeolog antikoerper, mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) antikoerper, DNA mismatch repair protein Mlh1 antikoerper, MLH1 antikoerper, Mlh1 antikoerper, mlh1.S antikoerper, mlh1 antikoerper, LOC588545 antikoerper
- Hintergrund
- The repair of mismatched DNA is essential to maintaining the integrity of genetic information over time. In bacteria the DNA repair process is accomplished by the MutL, MutH, and MutS proteins. The MutS protein initially recognizes and binds to mismatched DNA. Following this, MutH, an endonuclease, and MutL form a complex with MutS and carry out an excision repair mechanism. When bacteria are deficient in one of these enzymes a mutator phenotype arises characterized by genetic instability. The important role played by DNA repair enzymes is emphasized by the fact that they are highly conserved from bacteria to yeast to mammals. In yeast the proteins are called MutS homolog 2 (MSH2), MutL homolog (MLH1), and PMS1 which is also a homolog of MutL. MSH2 is involved in the initial recognition of mismatched nucleotides during the replication mismatch repair process. It is thought that after MSH2 binds to a mismatched DNA duplex it is joined by a heterodimer of MLH1 and PMS1 which together help facilitate the later steps in mismatch repair. Biochemical studies of the human homologs of DNA mismatch repair enzymes MLH1, PMS2, and MSH2 indicate that human MSH2 protein can bind mispaired DNA, and that human MLH1 and PMS2 can exist as a heterodimer. These and other studies support the conservation of eukaryotic DNA mismatch repair mechanisms. The G168-15 antibody recognizes human MLH1 (80-85 kDa). Full-length human recombinant MLH was expressed as a fusion protein, affinity purified, and used as immunogen.
- Molekulargewicht
- 80-85 kDa
- Pathways
- DNA Reparatur, Production of Molecular Mediator of Immune Response
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