ELISA. Western Blot: ATG5 antibody can be used for the detection of ATG5 at 1-2 μg/mL. Positive Control. Rat Spleen Tissue Lysate. Immunohistochemistry. Positive Control. Human Spleen Tissue Lysate. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Buffer
PBS containing 0.02 % Sodium Azide as preservative.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C
Informationen zur Lagerung
Store the antibody undiluted at 2-8 °C. Antibodies should not be exposed to prolonged high temperatures.
APG5 antikoerper, APG5-LIKE antikoerper, APG5L antikoerper, ASP antikoerper, hAPG5 antikoerper, ATG5 antikoerper, CG1643 antikoerper, DmAtg5 antikoerper, Dmel\\CG1643 antikoerper, atg5 antikoerper, 2010107M05Rik antikoerper, 3110067M24Rik antikoerper, AW319544 antikoerper, Apg5l antikoerper, Atg5l antikoerper, C88337 antikoerper, Paddy antikoerper, apg5l antikoerper, zgc:100934 antikoerper, ATATG5 antikoerper, AUTOPHAGY 5 antikoerper, MKP11.20 antikoerper, MKP11_20 antikoerper, autophagy related 5 antikoerper, Autophagy-related 5 antikoerper, ATG5 autophagy related 5 homolog (S. cerevisiae) antikoerper, autophagy related 5 L homeolog antikoerper, similar to S. cerevisiae ATG5 (YPL149W) which nucleates preautophagosome formation as a conjugate with Atg12 antikoerper, autophagy protein Apg5 family antikoerper, ATG5 antikoerper, Atg5 antikoerper, atg5 antikoerper, atg5.L antikoerper, APG5 antikoerper
Hintergrund
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG5, another member of the autophagy protein family, forms a conjugate with ATG12, this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy. This conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells. Three isoforms of ATG5 are known to exist, this ATG5 antibody will only detect the longest isoform.Synonyms: APG5-like, ASP, Apoptosis-specific protein, Autophagy protein 5