ATG5 Antikörper (N-Term)

Produktnummer ABIN1449605

Der Kaninchen Polyklonal anti-ATG5 Antikörper wird verwendet zum Nachweis von ATG5 in Proben von Human und Maus. Er wurde validiert für WB, IF, IHC (p) und EIA.

Kurzübersicht für ATG5 Antikörper (N-Term) (ABIN1449605)

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ATG5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-ATG5 Antikörper

Bindungsspezifität: AA 1-30, N-Term

Spezifität: This antibody recognizes Human and Mouse ATG5 (N-term). Other species not tested.

Aufreinigung: Saturated Ammonium Sulfate precipitation followed by dialysis against PBS

Immunogen: KLH conjugated synthetic peptide between 1~30 amino acids from the N-term of Human APG5L Genename: ATG5

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.25 mg/mL

Buffer: PBS containing 0.09 % (W/V) Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu ATG5

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Andere Bezeichnung: APG5L / ATG5

Hintergrund: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG5, required for autophagy, conjugates to ATG12 and associates with an isolation membrane to form a cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. APG5 may also play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.Synonyms: APG5-like, ASP, Apoptosis-specific protein, Autophagy protein 5

Molekulargewicht: 32447 Da

Gen-ID: 9474

NCBI Accession: NP_004840

Pathways: Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagie