MEK1 Antikörper (pThr291)

Produktnummer ABIN362381

Es sind 2+ Publikationen für dieses Produkt verfügbar. Der Kaninchen Polyklonal anti-MEK1 Antikörper wird verwendet zum Nachweis von MEK1 in Proben von Human, Maus und Ratte. Er wurde validiert für WB.

Kurzübersicht für MEK1 Antikörper (pThr291) (ABIN362381)

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MEK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-MEK1 Antikörper

Bindungsspezifität: pThr291

Spezifität: The antibody detects endogenous level of MEK1 only when phosphorylated at threonine 291.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pThr291 (P-R-T (p) -P-G derived from Human MEK1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 1:500-1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Referenzen für anti-MEK1 Antikörper (ABIN362381)

Xu, Zhao, Huang, Ding, Wang, Wang, Cheng, Kang, Wang: "CD40-expressing plasmid induces anti-CD40 antibody and enhances immune responses to DNA vaccination." in: The journal of gene medicine, Vol. 12, Issue 1, pp. 97-106, (2010) (PubMed).

Ikari, Atomi, Kinjo, Sasaki, Sugatani: "Magnesium deprivation inhibits a MEK-ERK cascade and cell proliferation in renal epithelial Madin-Darby canine kidney cells." in: Life sciences, Vol. 86, Issue 19-20, pp. 766-73, (2010) (PubMed).

Details zu MEK1

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Andere Bezeichnung: MEK1

Hintergrund: Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.

Molekulargewicht: 45 kDa

NCBI Accession: NP_002746

UniProt: Q02750

Pathways: MAPK Signalweg, RTK Signalweg, Interferon-gamma Pathway, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagie, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling