RAF1 (BRAF) (pSer642) Antikörper

Antigen: RAF1 (BRAF)

Epitope: pSer642

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Ratte (Rattus)

Applikation: Western Blot (WB)

Produktnummer: ABIN361491

Produktbeschreibung

Weitere Bezeichnung: Phospho-Ser642 Raf-1

Format: Affinity-purified

Beschreibung: The Ras pathway is a critical signal transduction cascade involved in regulating cellular proliferation, differentiation, survival, and oncogenic transformation. Members of the Raf serine/threonine kinase family are key intermediates in this cascade, functioning to relay signals from activated Ras to the downstream protein kinases MEK and ERK (Marshall, 1996). Previous studies have shown that phosphorylation is required for Raf-1 activation (Dhillon and Kolch, 2002, Chong et al., 2003). Recent work has demonstrated that phosphorylation also regulates the downregulation of Raf (Dougherty et al., 2005) with two sites participating: Ser301 and Ser642.

Spezifität: Specific for the ~74k Raf-1 protein phosphorylated at Ser642.

Anwendungen

Applikationshinweise: Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.

Reinigung: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and non-phosphopeptide affinity columns.

Lagerung: Store at -20 °C, stable for 1 year

Beschränkungen: Nur für Forschungszwecke einsetzbar