XIAP Antikörper (N-Term)

Produktnummer ABIN3044425

Produktdetails anti-XIAP Antikörper

Target: XIAP (X-Linked Inhibitor of Apoptosis (XIAP))

Bindungsspezifität: AA 14-34, N-Term

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser XIAP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for E3 ubiquitin-protein ligase XIAP(XIAP) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequenz: ADINKEEEFV EEFNRLKTFA N

Kreuzreaktivität (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Produktmerkmale: Rabbit IgG polyclonal antibody for E3 ubiquitin-protein ligase XIAP(XIAP) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: X-linked inhibitor of apoptosis
Protein Name: E3 ubiquitin-protein ligase XIAP

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human XIAP(14-34aa ADINKEEEFVEEFNRLKTFAN), different from the related mouse sequence by two amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Referenzen für anti-XIAP Antikörper (ABIN3044425)

Wei, Gao, Zheng, Zhang, Liu, Yang, Teng, Hou, Yin, Zou: "Protective Effects of Fucoidan on Aβ25-35 and d-Gal-Induced Neurotoxicity in PC12 Cells and d-Gal-Induced Cognitive Dysfunction in Mice." in: Marine drugs, Vol. 15, Issue 3, (2017) (PubMed).

Chen, Mao, Qiu, Liu: "Gene transfection mediated by polyethyleneimine-polyethylene glycol nanocarrier prevents cisplatin-induced spiral ganglion cell damage." in: Neural regeneration research, Vol. 10, Issue 3, pp. 425-31, (2015) (PubMed).

Li, Zhang, Fang, Yan, Zhao, Feng, Ma, Ye: "Aspirin overcomes Navitoclax-resistance in hepatocellular carcinoma cells through suppression of Mcl-1." in: Biochemical and biophysical research communications, Vol. 434, Issue 4, pp. 809-14, (2013) (PubMed).

Wu, Xi, Guo, Liu, Yang, Liu, Dong, Guo: "Exogenous ARC down-regulates caspase-3 expression and inhibits apoptosis of broiler chicken cardiomyocytes exposed to hydrogen peroxide." in: Avian pathology : journal of the W.V.P.A, Vol. 42, Issue 1, pp. 32-7, (2013) (PubMed).

Details zu XIAP

Target: XIAP (X-Linked Inhibitor of Apoptosis (XIAP))

Andere Bezeichnung: XIAP (XIAP Produkte)

Synonyme: API3 antikoerper, BIRC4 antikoerper, IAP-3 antikoerper, ILP1 antikoerper, MIHA antikoerper, XLP2 antikoerper, hIAP-3 antikoerper, hIAP3 antikoerper, api3 antikoerper, birc4 antikoerper, ilp1 antikoerper, miha antikoerper, xlp2 antikoerper, xxiap antikoerper, 1110015C02Rik antikoerper, Aipa antikoerper, Api3 antikoerper, Birc4 antikoerper, IAP3 antikoerper, ILP-1 antikoerper, mXIAP antikoerper, riap3 antikoerper, fb73d01 antikoerper, wu:fb73d01 antikoerper, zgc:91873 antikoerper, BIRC8 antikoerper, X-linked inhibitor of apoptosis antikoerper, X-linked inhibitor of apoptosis L homeolog antikoerper, XIAP antikoerper, xiap antikoerper, xiap.L antikoerper, Xiap antikoerper

Hintergrund: BIRC4, baculoviral IAP repeat-containing protein 4 is also known as XIAP(X-linked inhibitor of apoptosis protein). The BIRC4 gene comprises 6 exons. This gene is mapped to chromosome Xq25. This gene encodes a protein that belongs to a family of apoptotic suppressor proteins. Members of this family share a conserved motif termed, baculovirus IAP repeat, which is necessary for their anti-apoptotic function. This protein functions through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2 and inhibits apoptosis induced by menadione, a potent inducer of free radicals, and interleukin 1-beta converting enzyme. This protein also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7. Mutations in this gene are the cause of X-linked lymphoproliferative syndrome. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 2 and 11.

Synonyms: AP 13 antibody|API3 antibody|Apoptosis Inhibitor 3 antibody|Baculoviral IAP repeat containing 4 antibody|Baculoviral IAP Repeat Containing Protein 4 antibody|Baculoviral IAP repeat-containing protein 4 antibody|BIRC 4 antibody|BIRC4 antibody|E3 ubiquitin-protein ligase XIAP antibody|hIAP-3 antibody|hIAP3 antibody|HILP antibody|IAP 3 antibody|IAP like protein antibody|IAP-3 antibody|IAP-like protein antibody|IAP3 antibody|ILP 1 antibody|ILP antibody|ILP1 antibody|Inhibitor of apoptosis protein 3 antibody|Inhibitor of Apoptosis X Linked antibody|Mammalian IAP Homologue A antibody|MIHA antibody|X linked IAP antibody|X linked inhibitor of apoptosis antibody|X linked inhibitor of apoptosis protein antibody|X-linked IAP antibody|X-linked inhibitor of apoptosis protein antibody|Xiap antibody|XIAP_HUMAN antibody|XLP2 antibody

UniProt: P98170

Pathways: Apoptose, Caspase Kaskade in der Apoptose, Transition Metal Ion Homeostasis