IL-18 Antikörper (AA 37-193)

Produktnummer ABIN3044312

Produktdetails anti-IL-18 Antikörper

Target: IL-18 (IL18) (Interleukin 18 (IL18))

Bindungsspezifität: AA 37-193

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IL-18 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Interleukin-18(IL18) detection. Tested with WB, IHC-P, ICC in Human.

Marke: Picoband™

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Interleukin-18(IL18) detection. Tested with WB, IHC-P, ICC in Human.
Gene Name: interleukin 18 (interferon-gamma-inducing factor)
Protein Name: Interleukin-18

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human IL-18 recombinant protein (Position: Y37-D193). Human IL-18 shares 66% and 64% amino acid (aa) sequences identity with mouse and rat IL-18, respectively.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for IL-18 is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Referenzen für anti-IL-18 Antikörper (ABIN3044312)

Si, Zhang, Gao, Chen, Zhao, Cheng: "Effects of IFN-? on IL-18 Expression in Pregnant Rats and Pregnancy Outcomes." in: Asian-Australasian journal of animal sciences, Vol. 26, Issue 10, pp. 1399-405, (2014) (PubMed).

Wang, Zhang, Zhang, Xu, Fang: "Expression and localization of IL-18 in the hypothalamic-pituitary-ovarian axis of non-pregnant, pregnant, and abortive rats." in: Journal of reproductive immunology, Vol. 92, Issue 1-2, pp. 45-53, (2011) (PubMed).

Yang, Shen, Chen, Ge: "Role of interleukin 18 in acute lung inflammation induced by gut ischemia reperfusion." in: World journal of gastroenterology, Vol. 11, Issue 29, pp. 4524-9, (2005) (PubMed).

Details zu IL-18

Target: IL-18 (IL18) (Interleukin 18 (IL18))

Andere Bezeichnung: IL18 (IL18 Produkte)

Synonyme: Igif antikoerper, Il-18 antikoerper, IL-18 antikoerper, IGIF antikoerper, ChIL-18 antikoerper, IL-1g antikoerper, IL1F4 antikoerper, IL18 antikoerper, interleukin 18 antikoerper, Il18 antikoerper, IL18 antikoerper

Hintergrund: Interleukin-18, also known as IL18, is a protein which in humans is encoded by the IL18 gene. IL-18 is a cytokine produced by macrophages and other cells that belongs to the IL-1 superfamily. It is mapped to 11q22.2-q22.3. IL-18 works by binding to the interleukin-18 receptor, and together with IL-12, it induces cell-mediated immunity following infection with microbial products like lipopolysaccharide (LPS). After stimulation with IL-18, natural killer (NK) cells and certain T cells release another important cytokine called interferon-γ (IFN-γ) or type II interferon that plays an important role in activating the macrophages or other cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production in B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity.

Synonyms: Iboctadekin antibody|IFN gamma inducing factor antibody|IFN-gamma-inducing factor antibody|IGIF antibody|IL 1 gamma antibody|IL 18 antibody|IL 1g antibody|IL-1 gamma antibody|IL-18 antibody|IL1 gamma antibody|IL18 antibody|IL18 protein antibody|IL1F4 antibody|IL1g antibody|IL1gamma antibody|ILIF4 antibody|Interferon gamma inducing factor antibody|Interferon gamma-inducing factor antibody|Interleukin 1 gamma antibody|Interleukin 18 antibody|Interleukin-1 gamma antibody|Interleukin-18 antibody|Interleukin18 antibody|MGC12320 antibody

Gen-ID: 3606

UniProt: Q14116

Pathways: Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome