IL-18 Antikörper (Internal Region)

Produktnummer ABIN570987

Produktdetails anti-IL-18 Antikörper

Target: IL-18 (IL18) (Interleukin 18 (IL18))

Bindungsspezifität: Internal Region

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser IL-18 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Verwendungszweck: IL18

Sequenz: NPPDNIKDTK SDI

Spezifität: This antibody is expected to recognise both reported isoforms (NP_001553.1, NP_001230140.1).

Kreuzreaktivität: Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Güteklasse: Verified

Immunogen: Peptide with sequence C-NPPDNIKDTKSDI, from the internal region of the protein sequence according to NP_001553.1, NP_001230140.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 23 kDa band observed in lysates of cell line HeLa and approx. 21-22 kDa band in lysates of cell lines A431 and U2OS (calculated MW of 22.3 kDa according to NP_001553.1). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the golgi and cytoplasm of A431and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended conce

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu IL-18

Target: IL-18 (IL18) (Interleukin 18 (IL18))

Andere Bezeichnung: IL18 (IL18 Produkte)

Synonyme: Igif antikoerper, Il-18 antikoerper, IL-18 antikoerper, IGIF antikoerper, ChIL-18 antikoerper, IL-1g antikoerper, IL1F4 antikoerper, IL18 antikoerper, interleukin 18 antikoerper, Il18 antikoerper, IL18 antikoerper

Hintergrund: IL18, interleukin 18 (interferon-gamma-inducing factor), IGIF, IL-18, IL-1g, IL1F4, MGC12320, IL-1 gamma, interferon-gamma-inducing factor, interleukin 18, interleukin-1 gamma, interleukin-18

Gen-ID: 3606, 29197

NCBI Accession: NP_001553, NP_001230140

Pathways: Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome