MAPK11 Antikörper (Mitogen-Activated Protein Kinase 11) (N-Term)

Details for Product anti-MAPK11 Antibody No. ABIN2798348, Anbieter: Anmelden zum Anzeigen
Antigen
  • ATMPK11
  • F22L4.10
  • F22L4_10
  • MAP kinase 11
  • zgc:86905
  • P38B
  • P38BETA2
  • PRKM11
  • SAPK2
  • SAPK2B
  • p38-2
  • p38Beta
  • P38b
  • Prkm11
  • Sapk2
  • Sapk2b
  • p38beta
  • p38beta2
  • p38b
  • sapk2
  • prkm11
  • sapk2b
  • mitogen-activated protein kinase 11
  • MAP kinase 11
  • mapk11
  • LOC9328118
  • MPK11
  • MAPK11
  • Mapk11
  • PTRG_02565
Epitop
N-Term
17
17
16
15
13
12
9
8
7
7
6
6
5
4
4
3
2
2
1
1
1
Reaktivität
Maus
139
83
48
2
2
1
Wirt
Kaninchen
119
23
3
Klonalität
Polyklonal
Konjugat
Dieser MAPK11 Antikörper ist unkonjugiert
8
8
6
4
4
4
2
2
2
2
2
2
2
2
2
2
2
Applikation
Immunohistochemistry (IHC), Western Blotting (WB)
109
60
46
26
13
8
6
3
2
2
1
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
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'Independent Validation' Siegel
Antigen MAPK11
Chargennummer RAY8110713
Validierte Anwendung Western Blotting
Positivkontrolle primary mouse microglia
Negativkontrolle siRNA knock-down of MAPK11 in primary mouse microglia
Bewertung Passed. ABIN2798348 specifically recognizes Mapk11 in primary mouse microglia lysates.
Primärantikörper ABIN2798348
Sekundärantikörper anti-rabbit HRP-conjugated antibodies (DakoCytomation, P0448, lot 00055814)
Protokoll
  • Primary mouse microglia are grown in DMEM (Invitrogen), supplemented with 10% foetal bovine serum (Gibco), 10% horse serum and penicillin and streptomycin (Gibco), at 37°C and 5% CO2 in a 12-well dish.
  • Transfect cells with 0.05nmol mouse MAPK11 Silencer Select siRNA (ThermoFisher Scientific, Sample ID ASO10943, ASO1098J and ASO109CX, lot AMO04BXD) using Lipofectamine 3000 (Invitrogen) following the manufacturer´s instructions.
  • Lyse cells in 25µl per well cold lysis buffer (10mM Tris-HCl pH 8.0, 150mM NaCl, 1% Nonidet-P40, 1mM EDTA, 10mM NaF, 1mM Na3VO4, protease inhibitors (Sigma).
  • Determine total protein content of the lysates using Bradford assay (Bio-Rad, 500-0006, lot 111832).
  • Denature 5µg total protein for 5min at 95°C in 5µl Laemmli SDS sample buffer and subsequently separate them on a denaturing freshly cast 10% SDS-PAGE for 1h at 140V.
  • Transfer proteins onto PVDF membrane (Pall Life Sciences, 75696G, lot TO3225) with a semi-dry Western blotting system for 50min at 60mA.
  • Block the membrane with TBST (TBS, 0.1% Tween) containing 5% milk ON at 4°C.
  • Incubation with primary rabbit anti-MAPK11 antibody (antibodies-online, ABIN2798348, lot RAY8110713) diluted 1:500 in TBST containing 5% milk for 1h at RT.
  • Wash membrane 5x 5min with TBST.
  • Incubation with anti-rabbit horseradish peroxidase-conjugated secondary antibodies (DakoCytomation, P0448, lot 00055814) diluted 1:2000 in TBST containing 5% milk for 1h at RT.
  • Wash membrane 5x 5min with TBST and 3x 5min with TBS.
  • Reveal protein bands using ECL Prime Western Blotting Detection Reagent (GE Healthcare, RPN2232, lot 9547481) with Curix RP2 Plus medical X-ray films (AGFA Health Care, ENKMV, lot 79040074) on an AGFA Health Care Curix60-developer.
  • Strip membranes RestoreTM Western Blot Stripping Buffer (Thermo Scientific, 21059, lot NA 164269) and subsequently incubate with a GAPDH loading control antibody (Sigma, G9545, lot 049K4787) diluted 1:10000 and with anti-rabbit horseradish peroxidase-conjugated secondary antibodies (DakoCytomation, P0448, lot 00055814) diluted 1:2000.
  • Wash and reveal the protein bands using self-made ECL Western Blotting Detection Reagent with Curix RP2 Plus medical X-ray films (AGFA Health Care, ENKMV, lot 79040074) on an AGFA Health Care Curix60-developer.
Anmerkungen The MAPK11 antibody ABIN2798348 reveals a protein of the expected molecular weight of murine Mapk11 in lysates of primary mouse microglia. The protein band’s intensity decreases upon siRNA knockdown.
Validierungsbilder
Western Blotting validation image for anti-Mitogen-Activated Protein Kinase 11 (MAPK11) (N-Term) antibody (ABIN2798348) Primary mouse microglia were transfected with Mapk11 siRNA and Mapk11 was revealed us...
Isotyp Ig Fraction
Andere Bezeichnung Mapk11 (MAPK11 Antibody Abstract)
Molekulargewicht 41397 Da
Pathways MAPK Signalweg, Neurotrophin Signalübertragung, Activation of Innate immune Response, Response to Water Deprivation, Regulation of Muscle Cell Differentiation, ER-Nucleus Signaling, Hepatitis C, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling
Applikationshinweise Western Blot, Immunohistochemistry
Recommended Dilutions
WB: 1:1000, IHC: 1:10-50Western blot analysis of lysate from mouse NIH/3T3 cell line, using Mouse Mapk11 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Kommentare

Rabbit Anti-Mouse Mouse Mapk11 (N-term) Antibody, 400 μl

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 0.5 mg/mL
Lagerung 4 °C,-20 °C
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