TNFSF10 Antikörper (Tumor Necrosis Factor (Ligand) Superfamily, Member 10) (Biotin)

Details for Product anti-TNFSF10 Antibody No. ABIN2689130, Anbieter: Anmelden zum Anzeigen
Antigen
  • TRAIL
  • tl2
  • apo2l
  • cd253
  • trail
  • apo-2l
  • trail1
  • xtrail1
  • APO2L
  • Apo-2L
  • CD253
  • TL2
  • A330042I21Rik
  • AI448571
  • APO-2L
  • Ly81
  • Trail
  • tumor necrosis factor (ligand) superfamily, member 10
  • TNF-related apoptosis inducing ligand-like
  • TNFSF10
  • TRAIL-LIKE
  • tnfsf10
  • Tnfsf10
Reaktivität
Human
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3
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Wirt
Maus
191
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Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser TNFSF10 Antikörper ist konjugiert mit Biotin
37
21
14
10
5
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4
3
3
3
3
3
3
3
3
3
2
2
1
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1
Applikation
Flow Cytometry (FACS)
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49
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17
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10
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2
2
2
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Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Marke BD Pharmingen™
Immunogen Human TRAIL
Klon RIK
Isotyp IgG1
Produktmerkmale TRAIL (TNF-Related Apoptosis-Inducing Ligand), also known as Apo2L, is a member of the TNF ligand family. TRAIl is a type II membrane protein which may be expressed as a full-length, cell surface associated protein as well as in a soluble form. Both surface and soluble forms of TRAIL rapidly induce apoptosis on a wide range of cell lines. TRAIL has been shown to cause apoptotic death in either tumorigenic or transformed cells, but not in normal cells. TRAIL-mediated apoptosis has been shown to involve the activation of caspases, and is blocked by over-expression of the caspase-1 protease inhibitor, CrmA. TRAIL has also been reported to induce the transcription factor NF-κB in a cell type-specific manner. Two cognate TRAIL receptors DR4, and DR5, as well as two decoy receptors, DcR1/TRID and DcR2/TRUNDD have been identified. TRAIL has been shown to be involved in T cell cytotoxicity, but the exact physiological role TRAIL plays in T-cell mediated cytotoxicity remains to be elucidated. The RIK-2 antibody recognizes human TRAIL. Human TRAIL cDNA was transferred to an expression vector and transfected into the 2PK-3 mouse B cell lymphoma cell line to generate stable transfectants, which were then used to immunize mice. The RIK-2 clone was selected based on its ability to block cytotoxic activity. TRAIL has been renamed as CD253 recently. Flow cytometric analysis of TRAIL. Profile of human TRAIL/2PK-3 cell line analyzed on a FACScan™

BD Pharmingen™ Biotin Mouse Anti-Human CD253 - Biotin - Clone RIK-2 - Isotype Mouse IgG1 - Reactivity Hu - 0.2 mg
Reinigung The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Andere Bezeichnung CD253 (TNFSF10 Antibody Abstract)
Hintergrund Synonyms: TRAIL, APO-2L, TL2
Pathways Apoptose, Positive Regulation of Endopeptidase Activity
Applikationshinweise Flow Cytometry : Applications include flow cytometry (0.25-1.0 μg/1x10e6 cells). Blocking: The RIK-2 antibody is useful to block TRAIL-induced apoptosis. The no azide/low endotoxin format (NA/LE), Cat. No. 550912, is recommended for in vitro blocking assays.
Beschränkungen Nur für Forschungszwecke einsetzbar
Konzentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Lagerung 4 °C
Informationen zur Lagerung Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Produkt verwendet in: Kayagaki, Yamaguchi, Nakayama, Kawasaki, Akiba, Okumura, Yagita: "Involvement of TNF-related apoptosis-inducing ligand in human CD4+ T cell-mediated cytotoxicity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 5, pp. 2639-47, 1999 (PubMed).

Mariani, Matiba, Armandola, Krammer: "Interleukin 1 beta-converting enzyme related proteases/caspases are involved in TRAIL-induced apoptosis of myeloma and leukemia cells." in: The Journal of cell biology, Vol. 137, Issue 1, pp. 221-9, 1997 (PubMed).

Sheridan, Marsters, Pitti, Gurney, Skubatch, Baldwin, Ramakrishnan, Gray, Baker, Wood, Goddard, Godowski, Ashkenazi: "Control of TRAIL-induced apoptosis by a family of signaling and decoy receptors." in: Science (New York, N.Y.), Vol. 277, Issue 5327, pp. 818-21, 1997 (PubMed).

Pitti, Marsters, Ruppert, Donahue, Moore, Ashkenazi: "Induction of apoptosis by Apo-2 ligand, a new member of the tumor necrosis factor cytokine family." in: The Journal of biological chemistry, Vol. 271, Issue 22, pp. 12687-90, 1996 (PubMed).

Wiley, Schooley, Smolak, Din, Huang, Nicholl, Sutherland, Smith, Rauch, Smith: "Identification and characterization of a new member of the TNF family that induces apoptosis." in: Immunity, Vol. 3, Issue 6, pp. 673-82, 1996 (PubMed).

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