TRAIL Antikörper

Produktnummer ABIN1104738

Produktdetails anti-TRAIL Antikörper

Target: TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TRAIL Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Neutralization (Neut)

Marke: IHC-plus™

Spezifität: Human TRAIL/Apo2L

Aufreinigung: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human TRAIL/Apo2L.

Type of Immunogen: Cell extract

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.5 - 2 μg/mL), IHC, IHC-P (5 μg/mL), Neut (0.15 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: LS-B7420 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human sTRAIL/Apo2L (10 ng/mL), a concentration of 150 ng/mL of this antibody is required. ELISA: To detect hsTRAIL/Apo2L by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human sTRAIL/Apo2L (LS-C104497) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hsTRAIL/Apo2L. Western Blot: To detect hsTRAIL/Apo2L by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsTRAIL/Apo2L is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile water

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu TRAIL

Target: TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))

Andere Bezeichnung: TNFSF10 / TRAIL (TNFSF10 Produkte)

Synonyme: APO2L antikoerper, Apo-2L antikoerper, CD253 antikoerper, TL2 antikoerper, TRAIL antikoerper, A330042I21Rik antikoerper, AI448571 antikoerper, APO-2L antikoerper, Ly81 antikoerper, Trail antikoerper, tl2 antikoerper, apo2l antikoerper, cd253 antikoerper, trail antikoerper, apo-2l antikoerper, trail1 antikoerper, xtrail1 antikoerper, tnfsf10l2 antikoerper, zgc:92320 antikoerper, TNLG6A antikoerper, TNF superfamily member 10 antikoerper, tumor necrosis factor (ligand) superfamily, member 10 antikoerper, tumor necrosis factor superfamily member 10 antikoerper, TNF superfamily member 10 S homeolog antikoerper, TNFSF10 antikoerper, Tnfsf10 antikoerper, tnfsf10.S antikoerper, tnfsf10 antikoerper

Hintergrund: Name/Gene ID: TNFSF10
Family: TNF

Synonyms: TNFSF10, Apo-2L, APO2L, Apo-2 ligand, CD253, CD253 antigen, TRAIL, Protein TRAIL, TL2

Gen-ID: 8743

UniProt: P50591

Pathways: Apoptose, Positive Regulation of Endopeptidase Activity