TRAIL Antikörper

Produktnummer ABIN2689128

Produktdetails anti-TRAIL Antikörper

Target: TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser TRAIL Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS)

Marke: BD Pharmingen™

Produktmerkmale: The N2B2 antibody reacts with mouse TRAIL (TNF-Related Apoptosis-Inducing Ligand, also known as APO-2 ligand or Ly-81), a 40- kDa type-II transmembrane protein of the TNF family. This antibody recognizes mouse Trail-transfected 2PK-3 cells, but not the parental cell line. TRAIL selectively induces apoptosis in various transformed cells, but not in normal cells. Five receptors have been identified for human TRAIL, whereas only one receptor has been described for the mouse counterpart thus far. The pattern of expression of mouse TRAIL is controversial. TRAIL has been detected, with a rabbit polyclonal antibody, on the surface of activated CD4+ and CD8+ T lymphocytes, as well as on CD45R/B220+ lymphocytes, and transformed T- and B-cell lines. Similar results were obtained by western blot and mRNA analyses. However, these results could not be reproduced with the N2B2 mAb. TRAIL expression, detected with the N2B2 mAb, is induced on CD3- NK1.1+ cells after stimulation with IL-2 or IL-15. However, neither freshly isolated nor activated T cells showed TRAIL expression on the cell surface. Furthermore, TRAIL mediates NK-cell cytotoxicity, which is blocked by the N2B2 mAb. TRAIL has been renamed as CD253 recently.

BD Pharmingen™ Purified Rat Anti-Mouse CD253 - Purified - Clone N2B2 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.1 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse Trail-transfected 2PK-3 cells

Klon: N2B2

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-TRAIL Antikörper (ABIN2689128)

Degli-Esposti: "To die or not to die--the quest of the TRAIL receptors." in: Journal of leukocyte biology, Vol. 65, Issue 5, pp. 535-42, (1999) (PubMed).

Kayagaki, Yamaguchi, Nakayama, Takeda, Akiba, Tsutsui, Okamura, Nakanishi, Okumura, Yagita: "Expression and function of TNF-related apoptosis-inducing ligand on murine activated NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 4, pp. 1906-13, (1999) (PubMed).

Mariani, Krammer: "Surface expression of TRAIL/Apo-2 ligand in activated mouse T and B cells." in: European journal of immunology, Vol. 28, Issue 5, pp. 1492-8, (1998) (PubMed).

Mariani, Krammer: "Differential regulation of TRAIL and CD95 ligand in transformed cells of the T and B lymphocyte lineage." in: European journal of immunology, Vol. 28, Issue 3, pp. 973-82, (1998) (PubMed).

Details zu TRAIL

Target: TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))

Andere Bezeichnung: CD253 (TNFSF10 Produkte)

Synonyme: APO2L antikoerper, Apo-2L antikoerper, CD253 antikoerper, TL2 antikoerper, TRAIL antikoerper, A330042I21Rik antikoerper, AI448571 antikoerper, APO-2L antikoerper, Ly81 antikoerper, Trail antikoerper, tl2 antikoerper, apo2l antikoerper, cd253 antikoerper, trail antikoerper, apo-2l antikoerper, trail1 antikoerper, xtrail1 antikoerper, tnfsf10l2 antikoerper, zgc:92320 antikoerper, TNLG6A antikoerper, TNF superfamily member 10 antikoerper, tumor necrosis factor (ligand) superfamily, member 10 antikoerper, tumor necrosis factor superfamily member 10 antikoerper, TNF superfamily member 10 S homeolog antikoerper, TNFSF10 antikoerper, Tnfsf10 antikoerper, tnfsf10.S antikoerper, tnfsf10 antikoerper

Hintergrund: Synonyms: TRAIL, APO-2L, Ly-81, TL2

Pathways: Apoptose, Positive Regulation of Endopeptidase Activity