ATM Antikörper (pSer1981)

Produktnummer ABIN2688845

Produktdetails anti-ATM Antikörper

Target: ATM (Ataxia Telangiectasia Mutated (ATM))

Bindungsspezifität: pSer1981

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ATM Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), BioImaging (BI)

Marke: BD Pharmingen™

Produktmerkmale: ATM is the gene that is mutated in ataxia telangiectasia, an autosomal recessive hereditary disease that is characterized by delayed motor and sexual development, weakened immunity, multiple skin changes, increased sensitivity to ionizing radiation, and increased risk of developing certain cancers. The protein encoded by ATM is a phophoinositide-3-kinase-related protein kinase that is found in the nucleus and is responsible for early responses to the changes in chromatin structure caused by DNA double-strand breaks. Inactive wild type ATM protein forms homodimers or higher order multimers in which the C-terminal kinase domains are inhibited. It has been proposed that multimeric ATM is recruited to DNA double-strand breaks through interactions between its N-terminal HEAT domains and specific chromatin protein complexes. The inhibition of the kinase domains is lifted, resulting in auto-phosphorylation of the ATM at a single serine site and disruption of the multimers. The phosphorylated state of ATM is a rapid and highly sensitive indicator that cells have been exposed to agents that cause DNA double-strand breaks. The kinase domains of the resulting ATM monomers are accessible to a wide variety of substrates that are involved in DNA repair, cell-cycle regulation, and apoptosis. Thus ATM is a central regulator of cellular responses to ionizing radiation, and cells that lack ATM undergo radioresistant DNA synthesis and are resistant to γ radiation-induced apoptosis. The K88-534 monoclonal antibody recognizes human ATM phosphorylated at serine 1981 (S1981) and mouse ATM phosphorylated at its orthologous site, S1987. Immunofluorescent detection of ATM phosphorylation in human fibrosarcoma cells. HT-1080 cells (ATCC CCL-121) were seeded in a BD Falcon™ 96-well Imaging Plate (Cat. No. 353219) at ~ 10,000 cells per well. After overnight culture, DNA damage was induced by exposing the cells to bleomycin sulfate (250 units/mL) in serum-free medium for 2 hours at 37 °C (left panel) or the cells were untreated (right panel). To allow time for DNA repair processes to occur, the cells were washed, replenished with fresh complete medium, and returned to the incubator for 30 minutes. After treatment, cells were stained using the alcohol perm protocol and the Purified Mouse anti-ATM (pS1981) (pseudo-colored green), and counter-stained with Hoechst 33342 (pseudo-colored blue) according to the Recommended Assay Procedure. The second-step reagent was Alexa Fluor® 488 goat anti-mouse Ig (Invitrogen). Confocal images were captured on a BD Pathway™ 855 Bioimaging System using a 20x (0.75 NA) objective. Five sections, separated by 1 μm each, were captured and collapsed for viewing purposes using BD Attovision™ software. 560007 Rev. 1 Page 1 of 2

BD Pharmingen™ Purified Mouse anti-ATM (pS1981) - Purified - Clone K88-534 - Isotype Mouse IgG2b, κ - Reactivity Hu, Ms - 0.1 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Mouse ATM Peptide

Klon: K88

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: Confirmed by Bioimaging: Human Confirmed by western blot: Mouse

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-ATM Antikörper (ABIN2688845)

Pellegrini, Celeste, Difilippantonio, Guo, Wang, Feigenbaum, Nussenzweig: "Autophosphorylation at serine 1987 is dispensable for murine Atm activation in vivo." in: Nature, Vol. 443, Issue 7108, pp. 222-5, (2006) (PubMed).

Bakkenist, Kastan: "DNA damage activates ATM through intermolecular autophosphorylation and dimer dissociation." in: Nature, Vol. 421, Issue 6922, pp. 499-506, (2003) (PubMed).

Details zu ATM

Target: ATM (Ataxia Telangiectasia Mutated (ATM))

Andere Bezeichnung: ATM (ATM Produkte)

Synonyme: ATM antikoerper, Atm antikoerper, CG6535 antikoerper, Dmel\\CG6535 antikoerper, Tefu antikoerper, atm antikoerper, atm/tefu antikoerper, dATM antikoerper, tef antikoerper, Xatm antikoerper, at1 antikoerper, atdc antikoerper, tel1 antikoerper, telo1 antikoerper, AT1 antikoerper, ATA antikoerper, ATC antikoerper, ATD antikoerper, ATDC antikoerper, ATE antikoerper, TEL1 antikoerper, TELO1 antikoerper, AI256621 antikoerper, C030026E19Rik antikoerper, telomere fusion antikoerper, ATM serine/threonine kinase L homeolog antikoerper, ATM serine/threonine kinase antikoerper, ataxia telangiectasia mutated antikoerper, ataxia telangiectasia mutated (atm) antikoerper, serine/threonine-protein kinase ATM antikoerper, tefu antikoerper, atm.L antikoerper, atm antikoerper, ATM antikoerper, EDI_100660 antikoerper, CpipJ_CPIJ001772 antikoerper, BDBG_08252 antikoerper, PAAG_02532 antikoerper, MCYG_05088 antikoerper, VDBG_06833 antikoerper, ACLA_015700 antikoerper, LOC5565620 antikoerper, MGYG_07634 antikoerper, PGTG_14279 antikoerper, Atm antikoerper

Molekulargewicht: 349 kDa

Pathways: p53 Signalweg, Apoptose, DNA Reparatur, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus