EGF Antikörper (AA 45-275) (Biotin)
-
- Target Alle EGF Antikörper anzeigen
- EGF (Epidermal Growth Factor (EGF))
-
Bindungsspezifität
- AA 45-275
-
Reaktivität
- Schwein
-
Wirt
- Kaninchen
-
Klonalität
- Polyklonal
-
Konjugat
- Dieser EGF Antikörper ist konjugiert mit Biotin
-
Applikation
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Sequenz
- MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- APFLIF SHGNSIFRID LEGTNHEQLV ADAGISVLMD FHYNEERIYW VDLERQLLQR VFLNGTRQEK VCNLEKNVSG MAINWINEEL IWSNQQEGTI TVTDMKGNNS RVLLSALKYP ANVAVDPVER LMFWSSVVAG SLHRADVTGV EVRLLLETSE EIAAVSLDVL DKRLFWIQYN REGGSSRICS CDYDGGSVHF SKHLTQHNVF AMSLFGDHIF YSTWKKKTIW VANKH
- Spezifität
- It has been selected for its ability to recognize EGF in immunohistochemical staining and Western blotting.
- Aufreinigung
- Affinity Chromatography
- Immunogen
-
Recombinant EGF expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against EGF conjugated to biotin. - Isotyp
- IgG
- Top Product
- Discover our top product EGF Primärantikörper
-
-
- Applikationshinweise
-
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user. - Kommentare
-
Content: The quality control contains recombinant EGF (Ala45~His275) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % . - Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Format
- Liquid
- Konzentration
- Lot specific
- Buffer
- Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handhabung
- Avoid repeated freeze/thaw cycles
- Lagerung
- 4 °C
- Informationen zur Lagerung
- Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
- Haltbarkeit
- 12 months
-
- Target
- EGF (Epidermal Growth Factor (EGF))
- Abstract
- EGF Produkte
- Synonyme
- HOMG4 antikoerper, URG antikoerper, AI790464 antikoerper, CEGF antikoerper, epidermal growth factor antikoerper, pro-epidermal growth factor antikoerper, EGF antikoerper, egf antikoerper, CpipJ_CPIJ020278 antikoerper, Egf antikoerper
- Pathways
- NF-kappaB Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation
-