Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)
Sequenz
CGEIKDNKEL PQYLALTTR
Spezifität
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. Cross reactivity against CIITA from other sources may occur but has not been specifically determined.
Aufreinigung
Multi-step process.
Immunogen
Peptide corresponding to a region near the C-terminal end (amino acids 726-742) of the human CIITA gene conjugated to Keyhole Limpet Hemocyanin (KLH).
CIITA
Reaktivität: Human
WB, ELISA, IP
Wirt: Maus
Monoclonal
unconjugated
Applikationshinweise
Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation,conjugation and most immunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed by immunoblot against CIITAusing Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 1,000 is suggested for this product. Best Results are obtained using 0.03 % (v/v) Triton X-100 IN BUFFERS. Do not supplement buffers with Tween-20 detergent. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.0 mg/mL (by UV absorbance at 280 nm)
Buffer
0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 with 0.01 % Sodium Azide as preservative.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
-20 °C
Informationen zur Lagerung
Store original vial and reconstituted antibody at -20 °C.
Target
CIITA
(Class II, Major Histocompatibility Complex, Transactivator (CIITA))
CIITA antikoerper, C2ta antikoerper, EG669998 antikoerper, Gm9475 antikoerper, C2TA antikoerper, CIITAIV antikoerper, MHC2TA antikoerper, NLRA antikoerper, Mhc2ta antikoerper, class II, major histocompatibility complex, transactivator antikoerper, class II major histocompatibility complex transactivator antikoerper, MHC class II transactivator antikoerper, class II transactivator antikoerper, CIITA antikoerper, LOC710761 antikoerper, ciita antikoerper, LOC100443575 antikoerper, Ciita antikoerper, LOC100736732 antikoerper
Hintergrund
The transactivator CIITA regulates basal and interferon-induced expression of MHC class II genes. CIITA restores expression of all MHC II gene expression in mutant cells and corrects regulatory defects of those genes. Activation is via the proximal promoter. No DNA binding of in vitro translated CIITA was detected. CIITA may act in a coactivator-like fashion through protein-protein interactions by contacting factors binding to the proximal MHC class II promoter, to elements of the transcription machinery, or both. Alternatively it may activate HLA class II transcription by modifying proteins that bind to the MHC class II promoter.Synonyms: MHC class II transactivator, MHC2TA