CIITA Antikörper (AA 945-1130)

Produktnummer ABIN4886541

Produktdetails anti-CIITA Antikörper

Target: CIITA (Class II, Major Histocompatibility Complex, Transactivator (CIITA))

Bindungsspezifität: AA 945-1130

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CIITA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for MHC class II transactivator(CIITA) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for MHC class II transactivator(CIITA) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: class II, major histocompatibility complex, transactivator
Protein Name: MHC class II transactivator

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human CIITA recombinant protein (Position: E945-R1130). Human CIITA shares 84.4% amino acid (aa) sequence identity with mouse CIITA.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Referenzen für anti-CIITA Antikörper (ABIN4886541)

Sun, Huang, Yu, Zhang, Xu, Zhang, Li, Dong, Guo, Wang: "Autoregulatory loop between TGF-β1/miR-411-5p/SPRY4 and MAPK pathway in rhabdomyosarcoma modulates proliferation and differentiation." in: Cell death & disease, Vol. 6, pp. e1859, (2016) (PubMed).

Details zu CIITA

Target: CIITA (Class II, Major Histocompatibility Complex, Transactivator (CIITA))

Andere Bezeichnung: CIITA (CIITA Produkte)

Synonyme: CIITA antikoerper, C2ta antikoerper, EG669998 antikoerper, Gm9475 antikoerper, C2TA antikoerper, CIITAIV antikoerper, MHC2TA antikoerper, NLRA antikoerper, Mhc2ta antikoerper, class II, major histocompatibility complex, transactivator antikoerper, class II major histocompatibility complex transactivator antikoerper, MHC class II transactivator antikoerper, class II transactivator antikoerper, CIITA antikoerper, LOC710761 antikoerper, ciita antikoerper, LOC100443575 antikoerper, Ciita antikoerper, LOC100736732 antikoerper

Hintergrund: CIITA is a human gene which is mapped to 16p13. This gene encodes a protein with an acidic transcriptional activation domain, 4 LRRs (leucine-rich repeats) and a GTP binding domain. The protein is located in the nucleus and acts as a positive regulator of class II major histocompatibility complex gene transcription, and is referred to as the "master control factor" for the expression of these genes. Also, the protein binds GTP and uses GTP binding to facilitate its own transport into the nucleus. Once in the nucleus it does not bind DNA but rather uses an intrinsic acetyltransferase (AT) activity to act in a coactivator-like fashion. Mutations in this gene have been associated with bare lymphocyte syndrome type II (also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency), increased susceptibility to rheumatoid arthritis, multiple sclerosis, and possibly myocardial infarction. Several transcript variants encoding different isoforms have been found for this gene.

Synonyms: C2TA | CIITA | CIITA IV | MHC2TA | NLRA | P33076

Gen-ID: 4261

UniProt: P33076

Pathways: Cancer Immune Checkpoints