IL-6 Protein (AA 30-212)

Produktnummer ABIN7274962

Dieses Recombinant IL-6-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für IL-6 Protein (AA 30-212) (ABIN7274962)

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 90 % as determined by HPLC

Produktdetails

Proteineigenschaft: AA 30-212

Verwendungszweck: Human IL-6 Protein

Sequenz: Val30-Met212

Produktmerkmale: Recombinant Human IL-6 is expressed from E.coli without tag.It contains Val30-Met212.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 0.05 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human IL-6 at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-6 R alpha, hFc Tag with the EC50 32.6ng/ml determined by ELISA. The affinity constant of 0.22 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Andere Bezeichnung: IL-6

Hintergrund: IL-6 (Interleukin-6) is a pleiotropic cytokine that acts in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression.Interleukin 6 has been shown to interact with interleukin-6 receptor and glycoprotein.

Molekulargewicht: The protein has a predicted 20.8 kDa same as Tris-Bis PAGE result.

UniProt: P05231

Pathways: TLR Signalweg, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagie, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome