IFNA Protein (AA 28-436) (His-Avi Tag)

Produktnummer ABIN7274849

Recombinant IFNA-Protein exprimiert in HEK-293 Cells.

Kurzübersicht für IFNA Protein (AA 28-436) (His-Avi Tag) (ABIN7274849)

Target: IFNA (Interferon alpha (IFNA))

Protein-Typ: Recombinant

Spezies: Human

Quelle: HEK-293 Cells

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Produktdetails

Proteineigenschaft: AA 28-436

Aufreinigungstag / Konjugat: Dieses IFNA Protein ist gelabelt mit His-Avi Tag.

Verwendungszweck: Human IFN alpha/beta R1 Protein

Sequenz: Lys28-Lys436

Produktmerkmale: Recombinant Human IFN alpha/beta R1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Lys28-Lys436.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human IFNAR1, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-IFNAR1 Antibody, hFc Tag with the EC50 of 0.12μg/ml determined by ELISA. The affinity constant of 0.12 μM as determined in SPR assay (Biacore T200). See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: IFNA (Interferon alpha (IFNA))

Andere Bezeichnung: IFN alpha

Hintergrund: IFN-alpha / beta R1, also known as IFNAR1, belongs to the class II cytokine receptor family of proteins. Class II cytokine receptors form heterodimeric receptor complexes that mediate class II cytokine signals. Subunits of the different receptor complexes are shared and serve multiple functions.Functions in general as heterodimer with IFNAR2. Type I interferon binding activates the JAK-STAT signaling cascade, and triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT proteins and the IFNR alpha- and beta-subunits themselves.

Molekulargewicht: 50.2 kDa. Due to glycosylation, the protein migrates to 74-95 kDa based on Tris-Bis PAGE result.

UniProt: P17181-1

Pathways: JAK-STAT Signalweg, TLR Signalweg, Hepatitis C, Inflammasome