IL-6 Protein

Produktnummer ABIN6700154

Hersteller Produkt- Nr.: 010-001-310

Produktdetails

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Protein-Typ: Recombinant

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Hersteller Produkt- Nr.: 010-001-310

Hersteller: Rockland

Verwendungszweck: IL-6 Mouse Recombinant Protein

Aufreinigung: Purity greater than 95% as determined by reducing and non-reducing SDS-PAGE.

Reinheit: 95,00%

Endotoxin-Niveau: Measured by LAL is 0.05 EU/μg protein (with a 50% confidence range).

Biological Activity Comment: Recombinant mouse IL-6 is fully biologically active when compared to standards. The ED50, as determined by dose-dependent induction of 7TD1 cell proliferation, is 2.4-3.6 pg/mL.

Anwendungsinformationen

Applikationshinweise:

Other: User Optimized

Application_Note: Interleukin-6 (IL-6) has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-Interleukin-6 (IL-6) in immunological assays.

Kommentare:

Suggested_Applications: IHC, WB

Other_Performance_Data:

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Konzentration: 0.1 mg/mL

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

It is recommended to reconstitute the lyophilized recombinant M-IL-6 in 10 mM HCl to 0.1-1.0 mg/mL to regain full activity, this can then be further diluted to other aqueous solutions.

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Haltbarkeit: 6 months

Referenzen

Turnis, Kaminska, Smith, Kartchner, Vogel, Laxton, Ashmun, Ney, Opferman: "Requirement for antiapoptotic MCL-1 during early erythropoiesis." in: Blood, Vol. 137, Issue 14, pp. 1945-1958, (2021) (PubMed).

Antigendetails

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Andere Bezeichnung: Il6 (IL6 Produkte)

Hintergrund:

Synonyms: Interleukin-6 cytokine, IL-6, B-cell stimulatory factor 2, BSF-2, Interferon beta-2, IFN-beta-2, Hybridoma growth factor, CTL differentiation factor, CDF, Interleukin HP-1

Background: Interleukin (IL)-6, also know as BCDG, BCGF and BSF-2, is an important proinflammatory and immunoregulatory cytokine expressed by various cells. Interleukin-6 has been shown to inhibit the growth of early stage and to promote the proliferation of advanced stage melanoma cells in vitro. Recombinant Mouse IL-6 produced in E. coli is a single, non-glycosylated polypeptide chain containing 187 amino acids and having a molecular mass of 21,709 Daltons.

UniProt: P08505

Pathways: TLR Signalweg, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagie, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome