MEK1 Protein

Produktnummer ABIN5624610

Hersteller Produkt- Nr.: 009-001-gq3

Produktdetails

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Insect cells (Sf9)

Applikation: Western Blotting (WB), SDS-PAGE (SDS)

Hersteller Produkt- Nr.: 009-001-gq3

Hersteller: Rockland

Verwendungszweck: MEK1 human recombinant protein

Produktmerkmale: MAP2K, MEK, MEK1, MKK1, PRKMK1, MEK-1 recombinant protein

Aufreinigung: MEK1 is a recombinant protein containing a polyhistidine tag expressed in Sf9. Analysis by SDS-PAGE resulted in a pattern consistent with purified MEK1 and was estimated to be greater than 90% pure.

Sterilität: Sterile filtered

Anwendungsinformationen

Applikationshinweise:

Application Note: Human MEK1 recombinant protein has been tested in SDS-Page and is suitable as a control for polyclonal or monoclonal anti-MEK1 in immunological assays. For western blot use at 50 ng or less. For other assays concentration is user optimized. For a phosphorylated form of the protein co-expressed with constitutively active B-raf (V600E) see (p/n 009-001-GP5) MEK1 pS218-pS222.

Western Blot Dilution: 50 ng

Other: Dot Blot

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Antigendetails

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Andere Bezeichnung: MAP2K1 (MAP2K1 Produkte)

Hintergrund: Background: MEK1 recombinant protein was produced in Sf9 cells. Mitogen-activated protein kinase kinase 1, also known as MKK or MEK, is an integral component of the MAP kinase cascade that regulates cell growth and differentiation. This pathway also plays a key role in synaptic plasticity in the brain. Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase. MEK1 and MEK2 are about 80 % identical to each other, and nearly identical within the kinase domain. Recombinant MEK1 protein is ideal for investigators involved in Neuroscience, Cell Signaling and Cancer Research.

UniProt: Q02750

Pathways: MAPK Signalweg, RTK Signalweg, Interferon-gamma Pathway, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagie, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling