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MEK1 Protein (double Mutant)

MAP2K1 Spezies: Human Wirt: Insect cells (Sf9) Recombinant WB, SDS
Rockland
Produktnummer ABIN5624609
Hersteller Produkt- Nr.: 009-001-gq6
  • Target Alle MEK1 (MAP2K1) Proteine anzeigen
    MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    double Mutant
    Spezies
    • 8
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Quelle
    • 6
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Insect cells (Sf9)
    Applikation
    Western Blotting (WB), SDS-PAGE (SDS)
    Hersteller Produkt- Nr.
    009-001-gq6
    Hersteller
    Rockland
    Verwendungszweck
    MEK1 double mutant human recombinant protein
    Produktmerkmale
    MAP2K, MEK, MEK1, MKK1, PRKMK1, MEK-1 recombinant protein
    Aufreinigung
    MEK1 double mutant human is a recombinant protein containing a polyhistidine tag expressed in Sf9. This double mutant protein contain alanine substitutions at S218A and S222A rendering it inactive. Analysis by SDS-PAGE resulted in a pattern consistent with purified MEK1 and was estimated to be greater than 90% pure.
    Sterilität
    Sterile filtered
    Top Product
    Discover our top product MAP2K1 Protein
  • Applikationshinweise

    Application Note: Human MEK1 mutant recombinant protein has been tested in SDS-Page and is suitable as a negative control protein for immunoassays using antibodies targeting the critical MEK1 phosphorylated S218 or S222 residues. For western blot use at 50 ng or less. For other assays concentration is user optimized.

    Western Blot Dilution: 50 ng

    Other: Dot Blot

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
    Haltbarkeit
    12 months
  • Target
    MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))
    Andere Bezeichnung
    MAP2K1 (MAP2K1 Produkte)
    Hintergrund

    Synonyms: MAP2K, MEK, MEK1, MKK1, PRKMK1, MEK-1 recombinant protein

    Background: MEK1 isoform double mutant recombinant protein is modified to put alanine residues at two key activation sites, Serine 218 and Serine 222. Mitogen-activated protein kinase kinase 1, also known as MKK or MEK, is an integral component of the MAP kinase cascade that regulates cell growth and differentiation. This pathway also plays a key role in synaptic plasticity in the brain. Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase. The inactive double mutant MEK1 recombinant protein is ideal for investigators involved in Neuroscience, Cell Signaling and Cancer Research.

    UniProt
    Q02750
    Pathways
    MAPK Signalweg, RTK Signalweg, Interferon-gamma Pathway, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagie, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling
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