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AKT2 Protein

AKT2 Spezies: Human Wirt: Baculovirus infected Insect Cells Recombinant WB
Produktnummer ABIN5624570
  • Target Alle AKT2 Proteine anzeigen
    AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))
    Protein-Typ
    Recombinant
    Spezies
    • 11
    • 3
    • 1
    • 1
    Human
    Quelle
    • 5
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    Baculovirus infected Insect Cells
    Applikation
    Western Blotting (WB)
    Aufreinigung
    Recombinant protein corresponds to amino acids 1 to 479 of mature human AKT2, Akt isoform 2. The recombinant protein contains a polyhistidine affinity tag at the amino terminus. This protein was co-expressed with the p110 kinase domain leading to phosphorylation of key residues T308 and S473. Purity is greater than 90% as determined by reducing and non-reducing SDS-PAGE and by analytical HPLC.
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    Discover our top product AKT2 Protein
  • Applikationshinweise

    Application Note: AKT2 is suitable as a control for polyclonal or monoclonal anti-AKT2 in immunological assays. Akt2 recombinant protein is expected to be phosphorylated and in an active state. It is well suited as a control for anti-AKT pT308 which detects phosphorylated T308 residue, and anti-AKT pS473, that detects phosphorylated S473. For western blot use at 50 ng or less. For other assays concentration is user optimized.

    Western Blot Dilution: 50 ng

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1.09 mg/mL
    Buffer

    Buffer: 20 mM Tris pH 8, 300 mM NaCl with 10 % glycerol

    Stabilizer: 10 % (v/v) Glycerol

    Konservierungsmittel
    Without preservative
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Store vial at -70° C prior to use. Thaw only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. For long term storage we recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
    Haltbarkeit
    12 months
  • Target
    AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))
    Andere Bezeichnung
    AKT2 (AKT2 Produkte)
    Synonyme
    AKT2 Protein, pkbb Protein, pkbbeta Protein, pkbss Protein, prkbb Protein, rac-beta Protein, v-akt2 Protein, 2410016A19Rik Protein, AW554154 Protein, PKB Protein, PKBbeta Protein, HIHGHH Protein, PKBB Protein, PKBBETA Protein, PRKBB Protein, RAC-BETA Protein, cb945 Protein, akt2-a Protein, AKT serine/threonine kinase 2 Protein, v-akt murine thymoma viral oncogene homolog 2 Protein, thymoma viral proto-oncogene 2 Protein, v-akt murine thymoma viral oncogene homolog 2 L homeolog Protein, AKT2 Protein, akt2 Protein, Akt2 Protein, akt2.L Protein
    Gen-ID
    208
    UniProt
    P31751
    Pathways
    PI3K-Akt Signalweg, RTK Signalweg, AMPK Signaling, TLR Signalweg, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C, VEGF Signaling
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