V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) Protein

Details zu Produkt Nr. ABIN5624569, Anbieter: Anmelden zum Anzeigen
Proteinname
  • AKT2
  • pkbb
  • pkbbeta
  • pkbss
  • prkbb
  • rac-beta
  • v-akt2
  • 2410016A19Rik
  • AW554154
  • PKB
  • PKBbeta
  • HIHGHH
  • PKBB
  • PKBBETA
  • PRKBB
  • RAC-BETA
  • cb945
  • akt2-a
  • AKT serine/threonine kinase 2
  • v-akt murine thymoma viral oncogene homolog 2
  • thymoma viral proto-oncogene 2
  • v-akt murine thymoma viral oncogene homolog 2 L homeolog
  • AKT2
  • akt2
  • Akt2
  • akt2.L
Spezies
Human
20
2
1
1
Quelle
Baculovirus infected Insect Cells
9
6
4
2
1
1
1
Protein-Typ
Recombinant
Applikation
Western Blotting (WB)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Reinigung Recombinant protein corresponds to amino acids 1 to 479 of mature human AKT2, Akt isoform 2. The recombinant protein contains a polyhistidine affinity tag at the amino terminus. Purity is greater than 90% as determined by reducing and non-reducing SDS-PAGE and by analytical HPLC.
Proteinname
Gen-ID 208
UniProt P31751
Pathways PI3K-Akt Signalweg, RTK Signalweg, AMPK Signaling, TLR Signalweg, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C, VEGF Signaling
Applikations-hinweise

Application Note: AKT2 (phosphatase treated) is suitable as a control for polyclonal or monoclonal anti-AKT2 in immunological assays. Akt2 recombinant protein is dephosphorylated and in an inactive state. It is well suited as a control for anti-AKT pT308 which detects phosphorylated T308 residue, and anti-AKT pS473, that detects phosphorylated S473. For western blot use at 50 ng or less. For other assays concentration is user optimized.

Western Blot Dilution: 50 ng

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1.17 mg/mL
Buffer

Buffer: 20 mM Tris pH 8, 300 mM NaCl with 10 % glycerol

Stabilizer: 10 % (v/v) Glycerol

Konservierungs-mittel Without preservative
Lagerung -20 °C
Informationen zur Lagerung Store vial at -70° C prior to use. Thaw only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. For long term storage we recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
Haltbarkeit 12 months
Bilder des Herstellers
SDS-PAGE (SDS) image for V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) protein (ABIN5624569) SDS-PAGE of AKT2 (phosphatase treated) Human Recombinant Protein. Lane 1: AKT2, unred...
Western Blotting (WB) image for V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) protein (ABIN5624569) Western Blot of AKT2 (phosphatase treated) Human Recombinant Protein. Lane 1: SuperSi...
Western Blotting (WB) image for V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) protein (ABIN5624569) Western Blot of Rabbit AKT Antibodies. Lane 1: NIR MW protein ladder. Lane 2: AKT1, r...
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