IL-1 beta Protein (AA 118-269)

Produktnummer ABIN2018012

Dieses Recombinant IL-1 beta-Protein wird in CHO Cells exprimiert.

Kurzübersicht für IL-1 beta Protein (AA 118-269) (ABIN2018012)

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: CHO Cells

Reinheit: > 95 % as analyzed by SDS-PAGE and HPLC.

Produktdetails

Proteineigenschaft: AA 118-269

Produktmerkmale: ED50 < 10 pg/mL, measured in a cell proliferation assay using D10S cells, corresponding to a specific activity of > 1×10^8 units/mg.

Endotoxin-Niveau: < 0.2 EU/μg, determined by LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstituted in ddH2O or PBS at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Lagerung: -80 °C

Informationen zur Lagerung: Lyophilized recombinant Murine Interleukin 1 beta (IL-1 beta) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rmIL-1beta should be stable up to 1 week at 4 °C or up to 2 months at -20 °C.

Haltbarkeit: 6 months

Antigendetails

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Andere Bezeichnung: Interleukin 1 beta (IL-1 Beta)

Hintergrund: Interleukin-1 (IL-1) is a family of cytokines that play a central role in the regulation of immune and inflammatory responses to infections or sterile insults. IL-1a and IL-1b are the first two members discovered in this family, which are the products of distinct genes recognizing the same cell surface receptors. IL-1a and IL-1b are structurally related polypeptides that show approximately 25 % homology at the amino acid level. Both proteins are produced by a wide variety of cells in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins. The proteins are synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17.5 kDa. The specific protease responsible for the processing of IL-1b is interleukin 1b-converting enzyme (ICE)/caspase-1. Mature human and mouse IL-1b share approximately 75 % amino acid sequence identity where human IL-1b has been found to be active on murine cell lines. Interleukin (IL)-1b, murine, produced in CHO cells, is a non-glycosylated polypeptide chain containing 152 amino acids and having a molecular mass of 17,400 Da.
Synonyms: Catabolin, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF)

Molekulargewicht: 17.4 kDa, observed by non-reducing SDS-PAGE.

UniProt: P10749

Pathways: NF-kappaB Signalweg, Interferon-gamma Pathway, TLR Signalweg, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagie, Cancer Immune Checkpoints, Inflammasome